doi:10.1006/exnr.2001.7792, available online at http://www.idealibrary.com on
Effect of Oleamide on Sleep and Its Relationship to BloodPressure,Body Temperature, and Locomotor Activity in Rats
Salvador Huitro´n-Rese´ndiz,* Lhys Gombart,* Benjamin F. Cravatt,† and Steven J. Henriksen*
*Department of Neuropharmacology and
Department of Chemistry, The Scripps Research Institute, La Jolla, California 92037
Received April 12, 2001; accepted July 16, 2001
-9,10-octadecenoamide) is a brain lipidthat has recently been isolated from the cerebral ﬂuidof sleep-deprived cats. Intracerebroventricular andintraperitoneal administration of oleamide inducessleep in rats. However, it is unclear whether oleam-ide’s hypnogenic effects are mediated, in part, by itsactions on blood pressure and core body temperature.Here we show that systemic administration of oleam-ide (10 and 20 mg/kg) in rats increased slow-wave sleep2, without affecting blood pressure and heart rate. Inaddition, oleamide decreased body temperature andlocomotor activity in a dose-dependent manner. Theselatter effects were not correlated in time with the ob-served increases in slow-wave sleep. These data sug-gest that the hypnogenic effects of oleamide are notrelated to changes in blood pressure, heart rate, orbody temperature.
Oleamide and other alkane-chain fatty acid amides,including anandamide, the endogenous ligand for thecannabinoid receptor, constitute a novel group of ami-dated lipids that are normally found in the brain and blood of mammals, including humans (2, 7, 8, 30).Since its identiﬁcation and isolation, oleamide has been involved in diverse cellular and physiologicalfunctions.
, oleamide inhibits lymphocyte pro-liferation (26), potentiates the action of serotonin onsome receptor subtypes (5-HT
, and 5-HT
)(4, 23, 43,), modulates 5-HT
receptor-mediated effects(44), blocks gap junction communication (5, 17), and potentiates GABA
receptors (28, 29, 45, 46). On the