Welcome to Scribd, the world's digital library. Read, publish, and share books and documents. See more
Download
Standard view
Full view
of .
Look up keyword
Like this
1Activity
0 of .
Results for:
No results containing your search query
P. 1
Katayama Et.al. 97, Small Intestine, BBA

Katayama Et.al. 97, Small Intestine, BBA

Ratings: (0)|Views: 4 |Likes:
Uploaded from Google Docs
Uploaded from Google Docs

More info:

Published by: Matias Ceballos Guzman on Dec 09, 2010
Copyright:Attribution Non-commercial

Availability:

Read on Scribd mobile: iPhone, iPad and Android.
download as PDF, TXT or read online from Scribd
See more
See less

12/09/2010

pdf

text

original

 
 
This document contains text automatically extracted from a PDF or image file. Formatting may havebeen lost and not all text may have been recognized.
To remove this note, right-click and select "Delete table".
 
 
Ž Biochimica et Biophysica Acta 1347 1997 . 212–218
Distribution of anandamide amidohydrolase in rat tissues with
 
specialreference to small intestine
Kazuhisa Katayama
 b
, Natsuo Ueda
a
, Yuko Kurahashi
a
, Hiroshi Suzuki
a
,Shozo Yamamoto
a,)
, Itsuo Kato
 baDepartment of Biochemistry, Tokushima UniÕersity, School of Medicine, Kuramoto-cho, Tokushima 770, Japan bDepartment of CardioÕascular Surgery, Tokushima UniÕersity, School of Medicine, Kuramoto-cho, Tokushima 770, JapanReceived 25 March 1997; revised 6 May 1997; accepted 7 May 1997
AbstractAnandamide Ž arachidonylethanolamide . , an endogenous ligand for cannabinoid receptors, is hydrolyzed by anamidohy-drolase and its biological activity is lost. Previously, we partially purified the enzyme from porcine brain andanandamidesynthesis by its reverse reaction was proposed Ž Ueda et al., Ž 1995 . J. Biol. Chem. 270, 23823–23827 . . Theanandamidehydrolase and synthase activities were examined with various rat tissues. Rat liver showed the highest specificactivitiesŽ 4.4"0.3 and 4.5"0.5 nmolrminrmg protein . for the hydrolase and synthase, respectively. In most other tissues suchas brain, testis and parotid gland, the ratio of synthaserhydrolase activity was 0.7–1.6. However, small intestine showedarelatively high synthaserhydrolase ratio of about 5.0 Ž 1.0"0.1 and 0.2"0.1 nmolrminrmg protein . . When ahomogenateof small intestine was subjected to acetone extraction to remove lipids, a higher hydrolase activity was found Ž2.0"0.2nmolrminrmg protein . . Furthermore, Northern blotting detected an intense mRNA band of anandamide hydrolasein smallintestine as well as liver and brain. These results demonstrated for the first time a high content of anandamidehydrolase insmall intestine. q1997 Elsevier Science B.V.
Keywords: Anandamide; Cannabinoid; Arachidonic acid; Amidohydrolase; Small intestine; Rat
1. IntroductionAnandamide Ž arachidonylethanolamide . was foundas an endogenous ligand for cannabinoid receptors w 1x of either the brain type Ž CB1 . or the peripheral typeŽ CB2 . with a wide tissue distribution w 2 x . Hydrolysisof anandamide to arachidonic acid and ethanolamineresults in the loss of its biological activity. Thisreaction is catalyzed by an amidohydrolase the activ-ity of which was detected in brain, liver, eye and
Abbreviations: TLC, thin-layer chromatography.)Corresponding author. Fax: q81 886 33 6409.
several other tissues w 3–8 x . On the other hand, anan-damide can be formed by the condensation of arachi-donic acid with ethanolamine by ‘anandamide syn-

You're Reading a Free Preview

Download
/*********** DO NOT ALTER ANYTHING BELOW THIS LINE ! ************/ var s_code=s.t();if(s_code)document.write(s_code)//-->