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Biology Jun 2010 Actual Exam Paper Unit 3

Biology Jun 2010 Actual Exam Paper Unit 3

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Centre NumberCandidate NumberWrite your name here
SurnameOther names
 Total Marks
Paper Reference
Turn over 
*N35856A0116*
Edexcel GCE
Biology
Advanced SubsidiaryUnit 3B: Practical Biology and Research Skills
 Tuesday 11 May 2010 – Morning
Time: 1 hour 30 minutes
You must have:
Calculator
6BI07/01
Instructions
 
Use
black 
ink or ball-point pen.
 
Fill in the boxes
at the top of this page with your name,centre number and candidate number.
 
Answer
all
questions.
 
Answer the questions in the spaces provided
there may be more space than you need 
.
Information
The total mark for this paper is 40.
The marks for
each
question are shown in brackets
– use this as a guide as to how much time to spend on each question.
Advice
 
Read each question carefully before you start to answer it.
Keep an eye on the time.
Try to answer every question.
Check your answers if you have time at the end.
N35856A
©2010 Edexcel Limited.
1/1/1/1/1/
 
2
*N35856A0216*
BLANK PAGE
 
3
*N35856A0316*
Turn over 
 
Answer ALL questions.1
A student was interested in looking at some aspects of the efficiency of biologicalwashing powders. These powders contain protease enzymes.She found three sources of information about enzymes used in biological washingpowders.The first piece of information was about Savinase, which is made by a company calledNovozymes
®
.The extract below is taken from the Novozymes
®
website: 
Savinase is a cost-efficient protease, effective under most alkalineconditions and is especially effective under medium-temperature washconditions. Its optimal activity is at 55°C.
Further information, from a paper in the Archives of Dermatological Research in 1996,said:
In conclusion, the present study suggests that Savinase produces blisterson the skin.
The third source of information was from a National Centre for BiotechnologyEducation worksheet on washing-powder enzymes:
The enzymes are coated in a low melting-point wax and are therefore safeto handle and unlikely to form airborne dust. Unnecessary contact with thedissolved enzyme should be avoided. Wash splashes with plenty of water.Spills of enzyme solution should be wiped up promptly. 
Using this information, she designed some experiments. She investigated howmuch Savinase should be used to minimise cost but maximise efficiency in digestingprotein-based stains.Pieces of photographic film which had a layer of gelatine containing black particleswere used. The gelatine (a protein) was the substrate for the enzyme. As the gelatineis digested away, the black particles fall off and the film becomes clear.

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