--------------BIOCHEMISTRY--------------Biochemistry Laboratory – CH600 (2008-2009) Experiment 7
Characterization of Saponifiable Lipids
*Michelle Dy Sim, Gellina Ann Ram Suderio, Jonnah Kristina Chua Teope
Department of Biology, 3Biology-6, Group # 7, College of ScienceUniversity of Santo Tomas, España Street, Manila 1008
January 30, 2009
A saponifiable lipid is one with anester functional group that can behydrolyzedunder basic conditions. It undergoes
chemical hydrolysis when reacting with a strong base to produce salts of fatty acids called soaps. This experiment aims tocharacterize and to subject a fat or an oil sample to alkaline hydrolysis and to test the degree of unsaturation using iodine. For alkaline hydrolysis, 1g of NaOH was added with 2mL of butter / oil and 5mL of 95% ethanol then heated in a water bath at75
C for 15 minutes. Once cooled, a mongo size piece from the product was taken and submerged in 3mL distilled water.Using a straw, air was blown into it until bubbles started to form. The bubbles were able to persist for 1 minute and 35seconds for the coconut oil, 29 seconds for the vegetable oil and 1 minute for the butter. The remaining product was acidifiedwith a few drops of concentrated H
. The oily layer formed was acidic. For the acrolein test, 1g of KHSO
was placed in atest tube together with 5 drops of the butter sample then, heated on a hot plate, after cooling, the mixture showed a black precipitate that smelled like burnt wood. For the iodine test, 5mL of butter / oil sample was placed in a test tube. 5mL of water was placed in another test tube, this served as the control tube. To each test tube, iodine solution was carefully andslowly added, shaking it after each addition. The total number of drops added to each tube for the coconut oil was 100 drops,for the vegetable oil, 110 drops was used and for the butter, 32 drops.
Lipids are a variety of organic molecules grouped together on the basis of their solubility in non - polar solvents. Their varied biological functions include energy source, energy storage, cell membranestructural components, hormones, vitamins, vitamin adsorption, protection, and insulation.