Extraction & isolation method of phyto constituents (individual)
Means 'alkali like' hence resembles some characters like occurring complexamines i.e. containing one or more nitrogen atom.
Method -1:Powdered drugDefatted, moistened & render alkaline with Na
, NH3(Alkaloids are freed as bases)Extract with org solvent eg. CHCl
ether or methylene dichlorideTotal ExtractConcentrate & shake well with succesive quantities of inorganicacid.Residual organic fractionaqueous acid solution(Like pigment, fats, very weak bases or CHCl
soluble alkaloid Sulphates)(Alkaloidal salts)make alkaline & extractOrg solution of alkaloidal basesalkaloids with immiscibleremove solventsolvents(Crude alkaloid mixture) Purification by fractional Residual aquendescrystallization Chromatographic fractionseperation etc. Structure identification by modernanalytical techniques like U.V.,I.R, NMR, mass spectroscopy etc.