G. Veras et al. / Talanta 77 (2009) 1155–1159
In the present work, a portable, microcontrolled and low-cost spectrophotometer (MLCS) having a white LED as radiationsource and a CD media as diffraction grid for measurementsusing the visible region was developed. In order to increase thesignal-to-noise ratio in the spectrophotometric measurements, aphototransistor with maximum spectral sensitivity in the visibleregion was used. In the MLCS, pulsed radiation from the whiteLED is dispersed by the CD media generating monochromaticradiation that is focalized in the phototransistor by a steppermotor. The control of the proposed instrument, the acquisitionand treatment of data are accomplished by an electronic circuitbased on a programmable interrupt controller (PIC) microcon-troller. Two analytical applications were elected to illustrate theperformance of the MLCS. The ﬁrst is represented by individualdeterminations of four food colorants (tartrazine, brilliant blue,allura red and sunset yellow). The second concerns to the deter-mination of Fe
in restoratives by using the 1,10-phenantrolinemethod.
2.1. Reagents, solutions and samples
Food colorants tartrazine (E-102), sunset yellow (E-110), allurared (E-129), and brilliant blue (E-133) were purchased fromSigma–Aldrich. Stock solutions of 1000mgL
of each colorantwereprepareddissolvingitsnecessaryamountinphosphatebuffer
Schematic diagram illustrating the optical system of the MLCS.
solution at pH 7.00. Standard solutions of synthetic samples of each colorant were prepared by the appropriated dilution of therespective stock solution. The standard solutions were prepared inauthentic triplicates at the concentrations 2.0, 4.0, 6.0, 8.0, 10.0,12.0, 14.0, 16.0, 18.0, 22.0, and 26.0mgL
O was used to prepare stock solutions of Fe
. Working solutions (Fe
) were pre-pared from the stock solution after reduction of the Fe
usingascorbic acid 1.0% (w/v). Six samples of restorative oral solu-tions (containing Fe
) from different marks were acquired indrugstores at João Pessoa city, Brazil. These pharmaceutical for-
Electronic layout of the MCLS detector. Legend: PT=phototransistor; C=polyethylene capacitors; R=resistors; U1=operational ampliﬁer; U2=quadruple operationalampliﬁer; U3=quadruple switches. Details in text.