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METHODS- Baiting Techniques

METHODS- Baiting Techniques

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Published by Dom Gudez
mich! here's my part :)
mich! here's my part :)

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Categories:Types, School Work
Published by: Dom Gudez on Jan 22, 2011
Copyright:Attribution Non-commercial


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For the isolation and culture of zoosporic fungi using baiting techniques, baits wereobtained from dead insects like cockroaches. Aside from dead insects, shrimp exoskeleton ,snake skin, and pollen grains were also acquired for this experiment. This array of baitsincludes several types of keratin, some chitinous materials, and some cellulose or cellulosederivatives. The group were able to obtain three baits representing each of keratin, chitin andcellulose materials. Pond waters with soil or plant debris were also callected and placed insidesterile, deep-based petri dishes. Baits were placed on the pond water-filled petri dishes andincubated at room temperature under cool conditions. All baits were then examined under themicroscope after two days and daily after the first day of observation. The pollen grains wereexamined after 12 hours after culture and frequently thereafter. Plates were either directlyviewed under the scanning objective of microscope for culture observation. Samples werealso readily obtained by lowering a dry, clean cover slip, faced down onto the water surfaceand quickly withdrawn. The cover slip was then placed directly on glass slides with a drop ofwater and observed under the microscope. A small portion of the baits were also placed on aglass slide and was directly observed under the microscope. The hyphae, reproductivestructures, and zoospores were observed and these observations were recorded. For thepreservation of the materials, a drop of lactophenol mounting medium with acid fuchsin wasadded to the edge of the cover slip and was allowed to replace the water. The permanentmount was prepared and submitted to the laboratory instructors.

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