Science Project

Recommendations (9)


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(:)
At a recent meeting, members
discussed ways in which to determine which antioxidant
enzyme activities are induced for a particular exercise.
Antioxidants consume oxygen and free
radicals in large amounts in mammals. Individuals
exercising for extended periods can regulate the
antioxidant activity of their skeleton muscles, accentuating
the role that antioxidant plays in exercise.
However, identifying which antioxidant enzyme activities
are induced for a particular exercise is extremely difficult.
(NOTE : Add 2-4 sentences that describe characteristics of
the problem or statistics that reflect its severity)
, with a variation in effects of up to ten times.
The inability to identify a standardized
means makes it impossible to examine the exact role of
antioxidant enzyme activity in exercise.
(:)
Therefore, we recommend developing a method to assess
the role of antioxidant enzyme activity in which a power training protocol
can determine exact levels accurately. To do so,
exactly how power training influences the antioxidant enzyme activity of
skeleton muscles can be determined. Variations among antioxidant
enzyme methods can then be examined. Next, variations in power
training protocols can be examined. Additionally, the discriminative
features of skeleton muscles can be examined. As
anticipated, the proposed model can accurately determine antioxidant
enzyme activity, providing insight into how to increase muscular power.
Results of this study can contribute to efforts to manifest the
antioxidant ability in power training, thus increasing the rat skeleton
muscles markedly, which has significant physiological implications.
(NOTE : Add 2-4 more sentences that describe more thoroughly how the
results contribute to a particular field or sector)
(:)
Our senior management discussed recently concerns over
lost moisture and retrogradation of starch in foodstuffs. Given
that amylases that produce maltotriose as the major end product from raw
starch granules are relatively rare, efforts have been underway to optimize the
cultivation conditions for producing this extracellular amylase by Thermobifida
fusca NTU22. This newly isolated strain is of relevant interest
because the strain produces an extracellular amylase that releases maltotriose
as the major end product from either soluble starch or raw starch granules.
(NOTE : Add 2-4 sentences that describe characteristics of the problem or
statistics that reflect its severity) The commercially
produced form in bulk from microorganisms represents about 2533% of the
global enzyme market. Most _-amylases (EC 3.2.1.1, 1,4-_-d-glucan
glucanohydrolyase) produce glucose or maltose as the major product from
starch. Production of this extracellular amylase by maltotriose-producing -
amylase from Thermobifida fusca NTU22 can replace thermophilic
microorganisms. The inability to produce this
extracellular amylase by maltotriose-producing-amylase from Thermobifida
fusca NTU22 as a replacement for thermophilic microorganisms makes it
impossible to resolve problems involving lost moisture and retrogradation of
starch in foodstuffs.
(:)
Given that thermophilic microorganisms are
good sources of thermostability and novel amylases have
potential industrial importance, we recommend examining
the feasibility of producing this extracellular amylase by
maltotriose-producing-amylase from Thermobifida fusca
NTU22 substitute for thermophilic microorganisms.
To do so, a thermophilic actinomycete, Thermobifida
fusca NTU22 can be isolated from compost soils collected in
this study. A maltotriose-producing -amylase from
Thermobifida fusca NTU22 can then be purified from crude
culture filtrate by ammonium sulfate fractionation, Sepharose
CL-6B and DEAE-Sepharose CL-6B column
chromatography. Next, the overall yield of the purified
enzyme can be determined. Additionally, the purified enzyme
can produce SDS-polyacrylamide gel electrophoresis (SDS-
PAGE).
(:)
An anticipated, analysis results can indicate that, to
produce enzymes for enzymatic degradation of renewable
lignocellulose, a potent extracellular lignocellulolytic enzyme-
producing thermophilic actinomycete, Thermobifida fusca NTU22
from compost soils collected in Taiwan can be isolated. Results of
the -amylase gene from hermobifida fusca NTU22 can provide
further insight into the structure and the regulation of -amylase
production in this thermophilic actinomycete. In
addition to the commercial interest associated with producing high
yields of a specific maltooligosaccharide on degradation of starch
by -amylase, maltotriose has many excellent properties, including
a mild sweetness, an ability to store well in moisture, and anti-
retrogradation capabilities of starch in foodstuffs. (NOTE : Add 2
sentences that describe more thoroughly how the proposed
method contributes to a particular field or sector)
Further details can be found at
http://www.chineseowl.idv.tw