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DNA Fingerprinting BioRad

DNA Fingerprinting BioRad

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Published by gmundy
DNA fingerprinting technics
DNA fingerprinting technics

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Published by: gmundy on Feb 13, 2011
Copyright:Attribution Non-commercial


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Forensic DNA Fingerprinting KitInstruction Manual
Catalog #166-0007EDUexplorer.bio-rad.com
The kit is shipped at room temperature. Open immediately uponarrival and store reagent bag at –20°C within 3 weeks of receipt.
For technical service call your local Bio-Rad office, or in the US, call
Duplication of any part of this document is permitted for classroom use only.Please visit explorer.bio-rad.com to access our selection of language translations forBiotechnology Explorer kit curriculum.
Biotechnology Explorer
Can DNA evidence solve human problems?DNA fingerprinting is now used routinely to solve crimes. In recent years, news stories havereported how miniscule amounts of DNA have been used to identify individuals involved inincidents even many years in the past, as well as exonerate innocent people from incrimi-nation.The power of DNA as a tool for individual identification captures students’ imaginations.This activity provides in-depth instruction about how restriction enzymes cleave DNA, howelectrophoresis is used to separate and visualize DNAfragments, and how these techniquescan be combined to obtain a DNAfingerprint. Principles of restriction analysis, plasmidmapping and DNA fragment size determination can also be documented with this kit.Open the door to rich discussions about scientific, ethical, and legal implications of DNAprofiling. DNAfingerprinting is used in medical and forensic procedures, as well as inpaternity determinations to discern genetic relationships between individuals at the molecularlevel. This kit allows students to play the role of a forensic scientist and make a positiveID—that is, to simulate using real DNA as evidence and figure out for themselves: “Whodone it?”In this activity, students analyze six different samples of plasmid DNA. One sample collectedfrom a hypothetical “crime scene” and five samples obtained from “suspects” are digestedwith two restriction enzymes. The resulting DNAfragments are separated and visualized inagarose gels using Bio-Rad’s Fast Blast™ DNAstaining solution. Based on the restrictionfragment patterns, students compare the evidence and match one of the suspects’ DNA tothe sample collected at the crime scene.As an alternative to the classical human forensic applications for this kit, have your studentsimagine they are high tech pathologists investigating an outbreak of an aggressive infectiousdisease that has never been seen before. The Centers for Disease Control and Preventionsuspects that a new strain of bacteria has arisen that not only is the cause of the new disease,but also has acquired multiple resistance plasmids from some other bacterial strains. Their job is to develop a DNA diagnostic tool for identifying the culprit plasmids. They decide touse restriction enzyme analysis and “DNA electrophoresis fingerprinting” to identify anddistinguish different suspect plasmids and track their spread through the environment. DNAfrom the cultures of a number of stricken patients has been isolated. Have your studentsidentify the new killer bug before the pathogen gets out into the general population andstarts a true epidemic!We strive to continually improve our Biotechnology Explorer kits and curricula. Pleaseshare your stories, comments and suggestions!You can download this complete instruction manual on the Internet. Visit us on the Web atexplorer.bio-rad.com or call us in the US at 1-800-4BIORAD (1-800-424-6723).This curriculum was developed in collaboration with Len Poli and Russ Janigian of the S.F.Base Biotechnology Program in San Francisco, California, and Peggy Skinner of the BushSchool in Seattle, Washington. We’d like to thank them for their invaluable guidance andcontributions to this curriculum.Ron MardigianBiotechnology Explorer ProgramBio-Rad Life Science Group2000 Alfred Nobel DriveHercules, California 94547
Create context. Reinforce learning. Stay current.
New scientific discoveries and technologiescreate more content for you to teach,but not more time. BiotechnologyExplorer kits help you teach moreeffectively by integrating multiplecore content subjects into asingle lab. Connect conceptswith techniques and putthem into context withreal-world scenarios.
Environmentaland HealthScience
Epidemiology and diseaseGenetic testingRole, place, limits, andpossibilities of science andtechnologyPrivacy of information issuesCell structure and organizationTissue types for biologicalsamplingPlasmid mappingUse of restriction enzymes andelectrophoresis to fingerprint DNAUse of experimental controlsInterpretation of experimental resultsUse of DNA evidence in courtCreation and use of standard curvesRestriction fragment lengthpolymorphisms (RFLP analysis)Genetic engineering techniquesMendelian inheritancePlasmid mappingDNA structure, function,and chemistryChemistry of DNAelectrophoresisBiochemistry ofrestriction enzymesGenetic variations in thehuman genomeGenotype vs. phenotypeBiodiversityBacterial defensemechanisms
ScientificInquiryChemistryof LifeGeneticsCell andMolecularBiologyEvolution

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