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How Many Markers is Enough in Bone Marrow & Lymph Node Pathology

How Many Markers is Enough in Bone Marrow & Lymph Node Pathology

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Published by ninaradosevic2662
A very nice practical document to show you what to use in your everyday histodiagnostic practice
A very nice practical document to show you what to use in your everyday histodiagnostic practice

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Published by: ninaradosevic2662 on Feb 16, 2011
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01/30/2013

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How Many Markers isHow Many Markers isEnough?Enough?HematopathologyHematopathology
Emina Emilia Torlakovic, MD, PhDEmina Emilia Torlakovic, MD, PhDCollege of MedicineCollege of MedicineUniversity of SaskatchewanUniversity of SaskatchewanSaskatoon, SK, CanadaSaskatoon, SK, Canada
Kremer M, Quintanilla-Martinez L, Nahrig J, von Schilling C, Fend F.Immunohistochemistry in bone marrow pathology: a useful adjunctfor morphologic diagnosis.
Virchows Arch 
. 2005 Oct 18;:1-18
 
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IHC in BM pathologyIHC in BM pathology
 Acute leukemia (ALL and AML) Acute leukemia (ALL and AML)
Blasts estimates/countsBlasts estimates/counts
 – –MDS, MDS/MPN, MPNMDS, MDS/MPN, MPN
Malignant lymphomaMalignant lymphoma
Metastatic tumorsMetastatic tumors
Systemic mastocytosisSystemic mastocytosis
OtherOther
 Acute Leukemia Acute Leukemia
CD34, TdT, MPO, CD33, CD117, F8CD34, TdT, MPO, CD33, CD117, F8--ra,ra,CD61 (or CD42b), HgbCD61 (or CD42b), Hgb-- A, A,GlyGly--C, PaxC, Pax--5, CD10, CD79a, CD20, CD45, CD35, CD10, CD79a, CD20, CD45, CD3
To consider:To consider:NPM1, CD68, CD63,NPM1, CD68, CD63,lysozyme, CD15, CD123, CD99, CD31lysozyme, CD15, CD123, CD99, CD31
 
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MDS and MDS/MPNMDS and MDS/MPN
CD34, CD117, CD61 (or CD42b), MCTCD34, CD117, CD61 (or CD42b), MCT
To consider:To consider:MPO, CD33, MCT, HgbMPO, CD33, MCT, Hgb-- A, A,Parvovirus B19Parvovirus B19
Myeloproliferative NeoplasmsMyeloproliferative Neoplasms(MPN)(MPN)
CD34, MPO, CD61 (or CD42b or F8CD34, MPO, CD61 (or CD42b or F8--ra), PGra), PG--M1, HgbM1, Hgb-- A  A 
To consider:To consider:CD33, TdT, CD3, CD20,CD33, TdT, CD3, CD20,PaxPax--55

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