Firas A. – Biology Standard Level – IB1-A
65mL Yakult bottle, made in Almere
Salt solution, 0.9% sodium chloride
Petri dish, including MRS agar (jelly)
Extract exactly 1mL of Yakult from the bottle and place it in a test tube, using adropping pipette.2.Now it is the intention to produce 10mL of diluted Yakult. This can be done by adding9mL of distilled water to the existing 1mL of Yakult, placed in the test tube before.
Extract 1mL from the test tube to obtain 1mL of diluted Yakult. Take a new test tube.Further dilute the 1mL of diluted Yakult by adding another 9mL of distilled water tothe new test tube. 10mL of further diluted Yakult should be in the new test tube now.4.Repeat the above described process 5 times.
While conducting this process for the sixth time, extract 1mL of diluted Yakult and place it in a new test tube. In order to dilute this solution 1,000,000 times, add 9mL of the prepared salt solution (consisting of 0.9% sodium chloride) to the new test tube.
Extract exactly 1mL from the final diluted solution, using a dropping pipette. Placethis 1mL on a MRS agar (jelly substance) on a Petri dish, spreading the solution on theagar. This shall make sure that the bacteria are kept alive.7.Now the bacteria shall be given the chance to develop into colonies. This is done by placing the lid on the Petri dish, while keeping it in a plastic bag for three days. The plastic bag is important to avoid drying up of the Petri dishes.8.Now it is time to determine how many bacteria were present in the final solution. Thisis done by counting the number of bacteria colonies that developed in the Petri dish.