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Full Report for TLC

Full Report for TLC

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Published by Zak Magnifico

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Published by: Zak Magnifico on Mar 02, 2011
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03/02/2011

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GENERAL ANALYTICAL CHEMISTRYLABORATORY EXPERIMENTSSSC 1891
EXPERIMENT 4:SEPARATION OF ORGANIC DYES USING THINLAYER CHROMATOGRAPHY
INSTRUCTOR: PROF.MADYA HJ JAMIL YUSOF&ASSOC.PROF.JAFARIAH JAAFAR 
Prepared by:                                                                 Contributions:
LAU TIAN FWU                                                              33.33%ANG YEW SEE 33.33%CHIU SIEW LING      33.33%
 
Introduction
‡ Chromatography is method of separatingmixtures of two or more compounds‡ Distribute mixture between stationaryphase and mobile phase‡ Different compounds have differentsolubility and adsorption to the two phase
 
Introduction
‡ Thin layer chromatography (TLC) is asolid-liquid technique‡ Using two phase : stationary phase (solid)and mobile phase (liquid)‡ Solid are silica gel (SiO
2
.xH
2
O) andalumina (Al
2
O
3
.H
2
O)‡ Alumina more polar compare with silicagel

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