1.IdentifyIsland.exe tRNA-being-analysed.dat genome-being-compared_3I.dat GI-found_3I.

dat

2.IdentifyIsland.exe tRNA-being-analysed.dat genome-being-compared_3II.dat GI-found_3II.dat

3.IdentifyIsland.exetRNA-being-analysed.datgenome-being-compared_3III.dat GI-found_3III.dat

4.IdentifyIsland.exe tRNA-being-analysed.dat genome-being-compared_4.dat GI-found_4.dat

5.Run_TabulateIsland.bat

6.Prepare GI-found_checked.dat

Open GI_table.txt in output_data.

Values given sRNAs are noted and the corresponding data of each sRNA for the 4 genome taken

from the GI found_4.dat and manually prepare GI-found_checked.dat.

8.Run_ExtractFlank.bat

9.DNAnalyser.exe genome-being-compared_4.dat GI-found_checked.dat -o -g –z

10.The result of this from the terminal, copy , paste into theDNAanalyser.txt in the tRNAcc_win32 folder

http://archaea.ucsc.edu/cgi-bin/hgTracks

http://www.gene-profiles.org/gene/yjjg-sc4407-salmonella-enterica-subsp-enterica-serovar-
choleraesuis-str-sc-b67-3336965