Qualitative cyanide determination were carried out by Lorck method (7) modified by Alstrom (2). Isolates subcultured on NA medium were supplemented with glycine (4/4 gl-1). The production of cyanide was detected48h after inoculation, using picrate/Na2Co3 paper fixed to the under side of the Petri-dish lids which wherescaled with parafilm before incubation at 28
c. A change from yellow to orange, red, brown, or reddish brownwas recorded as an indication of weak, moderate, or strongly cyanogenic potential, respectively. Then,cyanogenic isolates were determined and identified. Among those, 4 pseudomonas fluorescent strains wereselected for further experiments.
volatile metabolites on root and shoot growth:
Pre-germinated surface sterilized wild barely, rye and wheat seeds (10 per plate) were placed equidistantlyapart on the surface of 1/0% agar. Rhizobacterial isolates were streaked on NA medium containing glycine andincubated for 24h. After incubation each inoculated plate was paired with a plate containing the pre-germinatedseeds (2).
liquid metabolites on root and shoot growth:A:
l of bacterial inoculations (the suspension of bacteria, nutrient broth medium, and glycine) were addedto the pre germinated and surface sterilized seeds which were placed on the surface of 1/0% agar (1).
cultures of each isolated strain of bacteria, grown for one day in nutrient broth medium (amended withglycine) were centrifuged at 10000 rpm for 6 min and 2ml of supernatant was added to the surface of 9/0%water agar plates. Fifteen pre germinated surface sterilized seeds of each weed species and wheat were thenplaced on each plate (8).Plates in all experiments were sealed with Parafilm and incubated in the dark at 27
c. Controls were consideredfor each experiment. Each isolate was tested in four replicates. After 48 hours the root and shoot length weremeasured. Data were analyzed using a one-way ANOVA.
Results and discussions:
The results of qualitative test of HCN production showed that low percent of isolated bacteria were capable of producing HCN (about 3.6%). Further tests indicated that the HCN producing isolates were belonging to
strains could produce HCN in different levels (out of 27 strains).Due to the results of semi-quantitative test of HCN, 4
super strains were identified as high HCNproducers.Analysis of the gas metabolite data showed that isolated bacteria significantly reduced the root and shootlength of weeds (P<0.01). According to the table1, root and shoot growth of weed seedlings were inhibited byvolatile metabolites during the growth of cyanogenic
in the paired-plate assemblies. This growthreduction in root length was 17 to 93.5% and in shoot length was 28 to 93% in comparison with those of controls.Table 1-Mean comparison of studied characters in wheat, rye grass And wild barley effected by volatilemetabolites produced by different
studied charactersBacterial treatments
root length % reduction shoot length % reduction
459 23.16 c 45.55 22.84 d 37.18
460 37.80 a 4.82 45.64 a -25.52
467 26.16 b 32.85 25.40 c 35.14
27-2 17.28 d 55.65 16.92 e 53.47Control
38.96 a 0 36.36 b 0LSD 1% 1.996 1.757
459 0.72 b 33.76 0.59 d 87.69
460 0.75 b 74.12 0.79 b 78.08
467 0.59 c 84.07 0.60 d 87.52
27-2 0.63 c 81.86 0.71 c 82.62Control
1.28 a 0 1.39 a 0LSD 1% 0.0495 0.0549