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Experimental Biochemistry

Experimental Biochemistry

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Published by Juan Pablo Bascur

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Published by: Juan Pablo Bascur on Mar 17, 2011
Copyright:Attribution Non-commercial

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02/22/2014

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CONTENTS
Section I Basic Techniques of Experimental Biochemistry 1Introduction 3Experiment 1 Photometry 15Experiment 2 Chromatography 25Experiment 3 Radioisotope Techniques 45Experiment 4 Electrophoresis 61Section II Proteins and Enzymology 79Introduction 81Experiment 5 Acid-Base Properties of Amino Acids 105Experiment 6 Sequence Determination of a Dipetide 111Experiment 7 Study of the Properties of ß-Galactosidase 123Experiment 8 Purification of Glutamate Oxaloacetate Transaminase from Pig Heart 135Experiment 9 Kinetic and Regulatory Properties of Aspartate Transcarbamylase 149Experiment 10 Affinity Purificaiton of Glutathione-S-Transferase 157Section III Biomolecules and Biological Systems 163Introduction 165Experiment 11 Microanalysis of Carbohydrate Mixtures by Isotopic Enzymatic, and ColorimetricMethods 195Experiment 12 Glcose-1-Phosphate: Enzymatic Formation from Starch and ChemicalCharacterization 205Experiment 13 Isolation and Characterization of Erythrocyte Membranes 217Experiment 14 Electron Transport 227Experiment 15 Strudy of the Phosphoryl Group Transfer Cascade Goferning Glucose MetabolismAllosteric and Covalent Regulation of Enzyme Activity 243Experiment 16 Experiments in Clinical Biochemistry and Metabolism 253Section IV Immunochemistry 261Introduction 263Experiment 17 Partial purification of a Polyclonal Antibody, Determination of Titer, andQuantitation of an Antigen Using the ELISA 279Experiment 18 Western Blot to Identify an Antigen 291Section V Nucleic Acids 301Introduction 303Experiment 19 Isolation of Bacterial DNA 333Experiment 20 Transformation of a genetic Character with Bacterial DNA 339Experiment 21 Constructing and Characterizing a Recombinant DNA Plasmid 345Experiment 22 In Vitro Transcription from a Plasmid Carrying a T7 RNA Polymerase-SpecificPromoter 359
 
Experiment 23 In Vitro Translation: mRNA tRNA, and Ribosomes 369Experiment 24 Amplification of a DNA Fragment Using Polymerase Chain Recation 385Section VI Information Science 397Introduction 399Experiment 25 Obtaining and Analyzing Genetic and Protein Sequence Information via the WorldWide Web, Lasergene, and RasMol 405Appendix Supplies and Reagents 409Index 437

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