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Production of Micro-Algal Concentrates for Aquaculture

Production of Micro-Algal Concentrates for Aquaculture

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Production of Micro-algal Concentrates for AquaculturePart 2:
 Sản xuất cô đặc Micro-tảo nuôi trồng thủy sản Phần 2:
Development and Evaluation of Harvesting, Preservation, Storage andFeeding Technology
 Phát triển và đánh giá của thu hoạch, bảo quản, lưu trữ và Công nghệ cho bé ăn
Michael Phillip Heasman, Tanya Maree Sushames,John Alfred Diemar, Wayne Andrew O’Connor andLynne Adele Foulkes.
NSW FisheriesPort Stephens Fisheries CentrePrivate Bag 1, Nelson Bay NSW 2315Australia
FRDC Project No. 93/123 & 96/342
August 2001
NSW Fisheries Final Report SeriesNo. 34ISSN 1440-3544
 
Table of 
Contents
TABLE OF CONTENTS
1.
NON
-
TECHNICAL SUMMARY
...........................................................................................................
III
2.
BACKGROUND
...................................................................................................................................
13.
NEED
.................................................................................................................................................
24.
OBJECTIVES
......................................................................................................................................
45.
RESEARCH METHODS
,
RESULTS AND DISCUSSION
...........................................................................
5
5.1. Selection of Micro-Algal
Species
 
.............................................................................................
55.2. An alternative to Bio-Assay for Evaluating Nutritional Quality of Stored Micro-AlgaeConcentrates.
............................................................................................................................
75.3. Micro-Algal Harvest and
Concentration...................................................................................
9
6.
SCREENING TRIALS USING INDIRECT CRITERIA TO EVALUATE PRESERVATION AND STORAGETECHNIQUES
...................................................................................................................................
23
6.1. Screening Trial 1: Preliminary Evaluation of Three
Additives...............................................
246.2. Screening Trial 2: Evaluation of Low Temperature Storage in Combination with Additives 296.3. Screening Trial 3: Evaluation of Additional
Additives...........................................................
446.4. Screening Trial 4: Evaluation of Additives across an Expanded Array of Micro-Algal
Species....................................................................................................................................
476.5. Screening Trial 5: Evaluation of Low Density Slurries under Modified Storage Conditions inExtending the Life of Concentrates of 
Pavlova lutheri
and Tahitian
Isochrysis
ProducedInitially as Pastes by
Super-Centrifugation
 
.............................................................................
566.6. Screening Trials 6: Evaluation of Frozen Storage of Micro-Algal
Concentrates....................
67
7.
BIO
-
ASSAY EXPERIMENTS TO EVALUATE ALTERNATIVE HARVEST
,
PRESERVATION
,
STORAGEAND FEEDING TECHNIQUES FOR CHILLED MICRO
-
ALGAL CONCENTRATES
.................................
73
7.1 Bio-Assay Experiment 1: Foundation Experiment to Define the Effect of Chilled StoragePeriod on Retained Nutritional Quality of a Standard Bivalve Mollusc Larval
Diet
Comprising Equal Amounts (on a dry weight basis) of 
Pavlova lutheri,
Tahitian
Isochrysis
and
Chaetoceros calcitrans
concentrated by
Super-Centrifugation........................................
737.2. Bio-Assay Experiment 2: Effects of Combinations of Three Types of Common FoodAdditives on the Shelf Life of 
Pavlova lutheri
as indicated by Direct Bio-Assay usingBivalve Larvae........................................................................................................................777.3. Bio-Assay Experiment 3: Evaluation of Optimal Harvesting Technique for 
Pavlovalutheri
(super centrifuge vs bucket centrifuge vs flocculation and stored as
slurry)
 
...............
817.4. Bio-Assay Experiment 4: Evaluation of Optimum Harvesting Techniques for Tahitian
Isochrysis
and
Thalassiosira pseudonana
..............................................................................
907.5. Bio-Assay Experiment 5: Evaluation of Optimum Harvesting Techniques for 
Chaetoceroscalcitrans
and
Skeletonema
costatum
 
...................................................................................
1037.6. Bio-Assay Experiment 6: Evaluation of Optimum Harvesting Techniques for 
Tetraselmischui
and
Chaetoceros
muelleri.
 
............................................................................................
1177.7. Bio-Assay Experiment 7: Evaluation of Optimum Stored Mono-Specific and Binary Dietsfor Sydney Rock Oyster 
Larvae............................................................................................
1267.8. Bio-Assay Experiment 8: Evaluation of Optimally Harvested and Stored Binary Dietsusing Juvenile Sydney Rock Oyster (
Saccostrea glomerata).
..............................................
132
8.
EFERENCES
................................................................................................................................ 1389.
B
ENEFITS
...................................................................................................................................... 14410. F
URTHER 
D
EVELOPMENT
............................................................................................................ 14511. C
ONCLUSIONS
.............................................................................................................................. 14612. A
PPENDICES
................................................................................................................................. 148
12.1.
Intellectual Property
.............................................................................................................
148
12.2.
 
Staff ......................................................................................................................................
148

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