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Table Of Contents

Pathogenesis of Proteus Infections
Genome Sequence Provides New Hints
Autotransporters: Simple yet Elegant
Functional Diversity of Autotransporters
Pta: Immunogenic Putative Serine Protease AT
Pta has No Bacterial Homologs
Cn3D Based Fold Prediction of Pta
Hypothesis
Various Forms of Pta Used in this Study
Purification and Initial Characterization in E. coli
Pta is 110 kDa Cell Surface-Associated AT
Interaction of E. coli-Pta with Hep2
Quantitative estimation of cell lysis
Kinetics of Bladder Cell Lysis by Pta
Qualitative Estimation of Bladder Cell Lysis
Characterization of the Purified Pta-α
Purification of the Passenger Domain: Pta-α
Protease Activity of Pta-α
Pta is an Alkaline Protease
Pta is a Subtilisin-like Alkaline Protease
Cytotoxicity Attributed to Protease Activity
Actin and Nuclear Damage by Pta
Role of Pta in P. mirabilis
Pta is an Inducible Autotransporter
Inactivation and Complementation of pta
Pta Contributes to Cytotoxicity in P. mirabilis
Pta Promotes Autoaggregation of P. mirabilis
Mutant Attenuated in Kidney and Spleen
Role for Pta in Virulence
Immunization with Pta*
Pta-Specific Ig Titer Correlates with Protection
Pta, a New Virulence Factor in P. mirabilis
Thanks are due to…
Wild-type Out-competes pta Strain in vivo
Approach to Identify Candidate Antigens
Immunization with Pta
Offers Moderate Protection
Proteus Infection: Irreversible Tissue Damage
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Pta Presentation

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Published by: Praveen Alamuri on Apr 06, 2011
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02/07/2013

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