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1040070138_TERM PAPER

1040070138_TERM PAPER

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Published by Vikal Rajput

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Published by: Vikal Rajput on Apr 14, 2011
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04/14/2011

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 LOVELYPROFESSIONALUNIVERSITY
TERM PAPER OF CONCEPTS INBIOTECHNOLOGY ON PRODUCTION OFRECOMBINANT PROTEIN IN EUKARYOTES
SUBMITTED TO,HARSH KUMAR.SUBMITTED BY,R109B44ACKNOWLEDGEMENT
I here by acknowledge that this term paper “PRODUCTION OF RECOMBINANTPROTEINS IN EUKARYOTES” has been made under the guidance of Mr. HarshKumar. Yet there is always room for improvement, I invite you for the improvement of this term paper.
 
CONTENTS
1. INTRODUCTON2. ADVANCES IN THE PRODUCTION OF HUMAN THEREUPEUTICPROTEINS IN YEAST AND FILAMENTOUS FUNGI3. RECOMBINANT PROTEIN PRODUCTION IN YEAST- EXPRESSION VECTORS
 
- FACTORS AFFECTING THE LEVEL OF RECOMBINANT PROTEINPRODUCTION4. PRODUCTION OF RECOMBINANT PROTEINS IN OTHER MICROPRGANISM5. PRODUCTION OF HUMAN FACTOR V|||6. ADVANTAGES OF USING ANIMAL CELL LINES FOR RECOMBINANTPROTEIN PRODUCTION7. OVER PRODUCTION OF RECOMBINANT PROTEIN- USE OF STRONG CONSTITUTIVE PROMOTER - INCLUSION OF AN INTRON- INCLUSION OF POLYDENYLATION SITE- REMOVAL OF UNNECESSARY AND UNTRANSLATED SEQUENCES- OPTIMIZATION OF TRANSGENE SEQUENCE FOR EFFICIENTTRANSLATION- INCORPORATION OF TARGETTING SIGNAL8. RESEARCH ON RECOMBINANT PROTEIN9. REFERENCE
INTRODUCTION
Recombinant DNA Technology involves the isolation and cloning of genes of interest,production of the necessary gene construts using appropriate enzymes and then transfer and expression of these genes into an appropriate host organism. This approach is also

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