USA and Canada United Kingdom and Ireland Germany All Other Countries
Tel 800 526 7319 UK Freephone 0800 622935 Freephone 0800 100 3496 Contact Your Local Distributornovatech@novagen.com Ireland Toll Free 1800 409445 firstname.lastname@example.org email@example.com firstname.lastname@example.org
pET System Manual
© 2005 EMD Biosciences, Inc., an affiliate of Merck KGaA, Darmstadt, Germany.The Novagen® name, Novagen logo, BugBuster®, His•Bind®, His•Tag®, HSV•Tag®, NovaTope®, Pellet Paint®, PopCulture®, and T7•Tag® are registered trademarks of EMD Biosciences, Inc. in the United States and certain other jurisdictions. Beta Red™, Clonables™, ColiRollers™, DsbA•Tag™, DsbC•Tag™, Ekapture™, FRETWorks™, GST•Bind™,GST•Mag™, GST•Tag™, His•Mag™, LIC Duet™, LumiBlot™, Lysonase™, Mobius™, Nova
™, Nus•Tag™, OnEx™, Overnight Express™, Perfect DNA™, Perfect Protein™, pETBlue™,pETcoco™, RoboPop™, Rosetta™, RosettaBlue™, Rosetta-gami™, S•Tag™, Singles™, SpinPrep™, Strandase™, Trail Mix™, TriEx™, Trx•Tag™, Tuner™, and Xarrest™ are trademarksof EMD Biosciences, Inc. Fractogel® and Benzonase® are registered trademarks of Merck KGaA, Darmstadt, Germany. MagPrep is a registered trademark of EMD Chemical, Inc.Sonifier® is a registered trademark of Branson Instruments, Inc. MacroPrep® is a registered trademark of Bio-Rad Laboratories, GmbH, Ltd. Cy5™ is a trademark of GE Health-care. Strep•Tactin® and Strep•Tag® are registered trademarks of IBA GmbH. PicoGreen® is a registered trademark of Molecular Probes, Inc. Triton® is a registered trademarkof Rohm and Haas Co. Superflow™ is a trademark of Sterogene Bioseparations Inc. SpeedVac® is a registered trademark of Thermo Savant, Inc. BugStopper™ is a trademark of Whatman International, Ltd.The pET expression system is covered under U.S. Patent Nos. 4,952,496; 5,693,489; and 5,869,320. For academic and non-profit laboratories, a nondistribution agreementaccompanies the products. Commercial laboratories must obtain a research-use license from Brookhaven Science Associates prior to purchase of the products.The pET-32 and pET-48 vectors are sold under patent license from Genetics Institute, Inc. For research use only. Licenses for commercial manufacture or use may be obtaineddirectly from Genetics Institute Inc., 87 Cambridge Park Drive, Cambridge, MA 02140. Vectors containing the His•Tag sequence are licensed under U.S. Patent Nos. 5,310,663;5,284,933; and European Patent No. 282,042 issued to Hoffmann-La Roche, Inc., Nutley NJ and/or Hoffmann, La Roche, Ltd. Basel, Switerzerland and are provided only for usein research. Information about license for commercial use is available from QIAGEN GmbH, Max-Volmer-Str. 4, D-40724 Hilden, Germany. Ni-NTA His•Bind Resins are manufac-tured by QIAGEN GmbH. His•Tag Monoclonal Antibody is covered under German Patent, No. DE 19 507 166 and international patent applications are filed under W09626963and is provided only for use in research. Information about license for commercial use is available from QIAGEN GmbH, Max-Volumer-Str. 4, D-40724 Hilden, Germany. TheGST•Tag technology is covered under U.S. Patent No. 5,654,176, European Patent No. 293,249B1, and Australian patent No. 607,511. Vectors containing a Strep•Tag sequencefor purification and detection are covered under U.S. Patent No. 5,506,121, U.K. Patent No. 2,272,698, and French Patent No. 93 13 066; Strep•Tactin is covered by U.S. PatentNo. 6,103,493 issued to IBA GmbH. Strep•Tactin resins, Strep•Tactin buffers, and Strep•Tag antibodies are manufactured by IBA GmbH. These products are provided only for usein research. Information about commercial use is available from IBA GmbH, Rudolf-Wissell-Str. 28, D-37079 Goettingen, Germany.Nova
™ and KOD DNA Polymerase are sold under licensing arrangements with F. Hoffmann La-Roche Ltd., and Roche Molecular Systems, Inc. KOD DNA Polymerases are man-ufactured by Toyobo and distributed by EMD Biosciences, Inc., Novagen brand. KOD products are not available through Novagen in Japan. KOD XL DNA Polymerase is licensedunder U.S. Patent No. 5,436,149 owned by Takara Shuzo, Co., Ltd. Purchase of KOD or Nova
DNA Polymerases is accompanied by a limited license to use it in the PolymeraseChain Reaction (PCR) process in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by the up-front license fee, either bypayment to Applied Biosystems or as purchased, i.e., an authorized thermal cycler.
TABLE OF CONTENTS