Today we're going to talk
about Sterilization, Disinfection, & Microbial Waste Disposal
,which is the main topic of this lecture. And about the first part
of non specific immunity
.So lets begin with some terms:
is the complete destruction of all microbes, including cells, spores, and viruses.And can be accomplished by physical methods such as radiation, heat. And can beaccomplished by chemical methods.In contrast to sterilization,
destruction or removal of all or most pathogensfrom objects by physical or chemical methods.
So sterilization is the destruction of all microbes, pathogenic or non pathogenic. Whiledisinfection only relates to pathogenic microorganisms
.An example of disinfection technique is basically wiping your skin with alcohol. It willremove most of microorganisms of it.Another example of disinfection is the process of pasteurization of liquids such as milk. So you heat the milk for a certain temperature for several times, and you do that to remove mainly brucella. We're not going to talk aboutanother micro organisms here, only brucella.So pasteurization, at the bottom line, is a type of disinfection technique.
are chemicals that we use to perform regular disinfections and usuallydisinfectants are used for non living objects such as bench tops.Disinfectants usually can killmost vegetative bacteria, most growing dividing bacteria, but cannot destroy regular sporesor bacterial spores.
, these are basically disinfectants that are safe enough to be used on living tissues.So 70% alcohol is a type of antiseptics, whereas bleach is a disinfectant because it's veryharmful for the skin, and if u put it on metals it might ruin the metals.Whenever you see a word that ends with –cide or –cidal, that basically means death so agermicide agent is an agent that kills germs.
is an agent that kills bacteria, but not necessarily bacterial spores.A
is an agent that kills bacterial spores.
A fungicidal agent
is an agent that kills fungi including their spores.
And Algicidal agents
is an agent that kills algae.
agent destroys viruses.We took this term for microbistatic, and
is an agent that inhibits growthand metabolism of bacteria, but not necessarily kill them. So if you remove the microbistaticagent from a microorganism, this microorganism can resume its growth.This is obviously a contrast
to bactericidal agent
that will actually kill the bacteria.Final two terms are sepsis and antisepsis.
efers to the presence of pathogen in blood or tissues, whereas
)means the absence of pathogens.