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Con. 2320-11. LR-9110 (3 Hours) [Total Marks : 75 N.B.: (1) All questions are compulsory. (2) All questions carry equal marks. Q.1 A) On which cellular organelle does intracellular digestion depend? And how it helps in cleaning the cell waste? What is the chemical content of those organelles? (10) B) Explain the association of dehydrogenase with NAD and NADP © OR ‘A) Describe the pathways which generate lipid second messengers 8) BB) Discuss molecular events involved in termination of transcription in bacteria a Q.2 A) In general which phase of the cell cycle has longer duration? Describe the three periods into which this phase is divided. (8) B) Explain the two maifi theories that explain the formation of Enzyme-substrate complex? (7) “OR ‘A)Explain different types of electron acceptor involved itvoxidative phosphorylation (7) BB) Discuss important properties of vectors in general and thei significance in gene TORR (@) 0.3 Explain Bioenergetics and thermodynamics. Describe the role of ATP in energy transfer during metabolism (15) oR Explain the properties of Plasmid which make them an important cloning ‘construction of genomic library and cDNA library. Add a note on the significance of these libraries (as) Q.4A) Explain the process of translation in eukaryotes (7) B) Discuss the mechanism of operation of voltage gated ion channels. (8)y OR 'A) Describe the modes of electron transfenin/biological oxidation-reduction reaction (7) B) Whatare:resttition endonucleases? Describe their role in DNA technology (8) Q.5 Write short notes on following (any three); A) Proteoglycans B) Gene splicing C) DNA gyrases and DNA ligases D)Chloroplast E) Apoptosis

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