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Table Of Contents

Nature of Light
The Spectrum of Microscopy
Wave Equations
Wavefront Propagation
Optical Path Length (OPL)
Laws of Reflection and Refraction
Total Internal Reflection
Evanescent Wave in Total Internal Reflection
Propagation of Light in Anisotropic Media
Multiple Wave Interference
Diffraction Grating
Useful Definitions from Geometrical Optics
Image Formation
Stops and Rays in an Optical System
Chromatic Aberrations
Spherical Aberration and Coma
Astigmatism, Field Curvature, and Distortion
Performance Metrics
The Compound Microscope
The Eye
Upright and Inverted Microscopes
The Finite Tube Length Microscope
Infinity-Corrected Systems
Telecentricity of a Microscope
Magnification of a Microscope
Numerical Aperture
Resolution Limit
Useful Magnification
Depth of Field and Depth of Focus
Köhler Illumination
Alignment of Köhler Illumination
Critical Illumination
Stereo Microscopes
Nomenclature and Marking of Objectives
Objective Designs
Special Objectives and Features
Special Lens Components
Cover Glass and Immersion
Common Light Sources for Microscopy
LED Light Sources
Polarizers and Polarization Prisms
The Selection of a Microscopy Technique
Image Comparison
Phase Contrast
Visibility in Phase Contrast
The Phase Contrast Microscope
Characteristic Features of Phase Contrast
Amplitude Contrast
Amplitude contrast
Oblique Illumination
Dark Field Microscopy
Optical Staining: Rheinberg Illumination
Optical Staining: Dispersion Staining
Shearing Interferometry: The Basis for DIC
DIC Microscope Design
Appearance of DIC Images
Reflectance DIC
Polarization Microscopy
Images Obtained with Polarization Microscopes
Confocal Microscopy
Scanning Approaches
Images from a Confocal Microscope
Configuration of a Fluorescence Microscope
Images from Fluorescence Microscopy
Properties of Fluorophores
Single vs Multi-Photon Excitation
Light Sources for Scanning Microscopy
Practical Considerations in LSM
Interference Microscopy
Interference microscopy
Optical Coherence Tomography/Microscopy
Optical Profiling Techniques
Optical Profilometry: System Design
Phase-Shifting Algorithms
Structured Illumination: Axial Sectioning
Stimulated Emission Depletion
4Pi Microscopy
The Limits of Light Microscopy
Raman and CARS Microscopy
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Field Guide of Microscopy

Field Guide of Microscopy

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Published by genio69

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Published by: genio69 on May 03, 2011
Copyright:Attribution Non-commercial


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