Welcome to Scribd, the world's digital library. Read, publish, and share books and documents. See more
Download
Standard view
Full view
of .
Save to My Library
Look up keyword
Like this
2Activity
0 of .
Results for:
No results containing your search query
P. 1
6th sem

6th sem

Ratings: (0)|Views: 746|Likes:
Published by Aman Bansal

More info:

Categories:Types, School Work
Published by: Aman Bansal on May 07, 2011
Copyright:Attribution Non-commercial

Availability:

Read on Scribd mobile: iPhone, iPad and Android.
download as DOCX, PDF, TXT or read online from Scribd
See more
See less

06/24/2011

pdf

text

original

 
RECOMBINANT DNA TECHNOLOGY
Course Code: BTB 601 Credit Units: 03Course Objective:
A complete understanding of molecular techniques can be obtained through the course. The successful applicationof biotechnology largely depends on these advanced molecular techniques.
Course Contents:
Module I: Enzymes used in RDT
Restriction endonuclease, methyltransferase, ligase, polymerase, kinase, phosphatase, nuclease, transferase, reversetranscriptase.
Module II: Cloning vectors
Plasmids, bacteriophages (Lambda and M13), phagemids, cosmids, artificial chromosomes (YAC, BAC). expressionvectors (Bacteria and yeast), vector engineering (fusion tags, antibiotic markers), codon optimization, hostengineering
Module III: Blotting techniques and hybridization
Southern, Northern and Western blotting techniques. Radioactive and non-radioactive probes.
Module IV: Nucleic acid amplification and its applicationsPrinciples
of PCR, designing of primers
Module V: Cloning Techniques
 Basic cloning experiment: Design of cloning strategy and stepwise experimental procedure , Complementation,colony and plaque hybridization, restriction, PCR, plus-minus screening, immunoscreening.
 Module VI: DNA Libraries
Purpose of constructing DNA libraries. Construction of cDNA and genomic libraries.
Module VII: Sequencing of DNA
DNA sequencing (Maxam Gilbert, Sanger¶s and automated), protein engineering.
Examination Scheme:
Components CT Attendance Assignment/Project/Seminar/QuizEEWeightage (%)
15 5 10 70
Text & References:
Text:
y
 
Principles of Gene Manipulation: An Introduction to Genetic Engineering, R.W. Old and S. B Primrose,Blackwell Science Inc.
y
 
Recombinant DNA, J.D. Watson et al, W.H. Freeman and Company.
 References:
y
 
Molecular Biotechnology: Principles and Applications of Recombinant DNA, B.R. Grick and J.J. Pasternak,ASM Press.
y
 
Molecular and Cellular Cells Methods in Biology and Medicine, P.B Kaufman, W. Wu, D. Kim and C.J. Cseke,CRC Press.
y
 
Milestones in Biotechnology: Classic Papers on Genetic Engineering, J.A. Bavies and W.S. Reznikoff,Butterworth Heinemann.
y
 
Gene Expression Technology, D.V. Goeddel in Methods in Methods in Enzymology, Academic Press Inc.
y
 
DNA Cloning: A Practical Approach, D.M. Glover and B.D. Hames, IRL Press.
y
 
Molecular Cloning: A Laboratory Manual, J. Sambrook, E.F. Fritsch and T. Maniatis, ColdSpringHarbor 
y
 
Laboratory Press.
 
ENZYMOLOGY AND ENZYME TECHNOLOGY
Course Code: BTB 602 Credit Units: 03Course Objective:
 
The course aims to provide an understanding of the principles and application of proteins, secondary metabolites andenzyme biochemistry in therapeutic applications and clinical diagnosis. The theoretical understanding of  biochemical systems would certainly help to interpret the results of laboratory experiments.
Course Contents:
Module I: Enzymes
 Introduction and scope, Nomenclature, Mechanism of Catalysis.
Module II: Enzyme Kinetics
 Single substrate steady state kinetics; Michaelis Menten equation, Linear plots, King-Altman¶s method; Inhibitorsand activators; Multisubstrate systems; ping-pong mechanism, Alberty equation, Sigmoidal kinetics and Allostericenzymes
Module III
Immobilization of Enzymes; Advantages, Carriers, adsorption, covalent coupling, cross-linking and entrapmentmethods, Micro-environmental effects.
ModuleIV: Enzyme reactors
 Reactors for batch/continuous enzymatic processing, choice of reactor type; idealized enzyme reactor systems, Masstransfer in enzyme reactors: Steady state analysis of mass transfer and biochemical reaction in enzyme reaction.
ModuleV: Bioprocess Design
 Physical parameters, reactor operational stability, Immobilized cells.
Module VI: Challenges and future trends
 Enzyme catalysis in organic media; catalytic antibodies and non protein biomolecules as catalysts, biocatalysts fromextreme thermophilic and hyper thermophilic Archae and Bacteria.
Examination Scheme:
Components CT Attendance Assignment/Project/Seminar/QuizEEWeightage (%)
15 5 10 70
Text & References:
Text:
 
y
 
Biotechnological Innovations in Chemical Synthesis, R.C.B. Currell, V.D. Mieras, Biotol Partners Staff,Butterworth Heinemann.
y
 
Enzyme Technology, M.F. Chaplin and C. Bucke, CambridgeUniversity Press.
y
 
Enzymes: A Practical Introduction to Structure, Mechanism and Data Analysis, R.A. Copeland, John Wiley andSons Inc.
 References:
 
y
 
Enzymes Biochemistry, Biotechnology, Clinical Chemistry, Trevor Palner.
y
 
Enzyme Kinetics: Behaviour and Analysis of Rapid Equilibrium and Steady State Enzyme Systems, I.H. Segel,Wiley-Interscience.
y
 
Industrial Enzymes & their applications, H. Uhlig, John Wiley and Sons Inc.
 
STRUCTURAL BIOLOGY
Course Code: BTB 603 Credit Units: 03
 
Course Objective:
The course aims to provide an understanding of the principles and applications of proteins, enzymes and nucleicacids for their role in biochemical pathway as well as interactions among themselves.
Course Contents:
Module I: Chemistry of amino acids and peptides
Side chain structure and function in protein folding and functionality: Secondary structure of proteins -helices,sheets, loops and turns; Structural and functional proteins. Tertiary structure of proteins, homo and hetero-dimers,trimers and tetramers; forces governing protein-protein interactions; open tertiary structure; Classification of  proteins; Structure and function of an antibody; structure of hemoglobin, muscle proteins; Sequence and structuralmotifs in proteins.
Module II: Protein-ligand interactions
Lock and key versus handshake mechanism of substrate recognition; structural basis of recognition; reactionmechanisms of enzymes, G-Protein coupled receptors.
Module III: Protein solubility, protein stability and stabilization
Salting in and salting out, Parameters affecting; enthalpic and entropic stabilization, mutations increasing stability,.helix capping; Native, partially denatured and denatured proteins; Protein denaturation, Physical and chemicaldenaturants; Refolding
Module IV: DNA structure
Covalent structure of DNA, base pairing, hydrogen bonding, DNA melting and annealing, difference between ATand GC pairing, DNA models, The Watson Crick model; Crystal structure of B-DNA, major and minor groves, dyadsymmetry, base pair stacking, propellor twist, A and Z- DNA, triple stranded DNA, telomeric sequences andstructure, G-quartcts, palindromic and tandem sequences, Base pair flipping and DNA bulges, DNA methylation;Protein-DNA interactions; drug-DNA interactions; Databases of sequences and structure for protein and DNA, public domain softwares for visualizing and modeling biomolecules -Rasmol, Deepview, Whatif.
Examination Scheme:
Components CT Attendance Assignment/Project/Seminar/QuizEEWeightage (%)
15 5 10 70
Text & References:
Text:
y
 
Biochemistry, R.H. Abeles, P.A. Frey and W.A. Jencks, Jones and Bartlett.
y
 
Essentials of Molecular Biology, D. Freifelder, Jones and Bartlett Publications.
y
 
Genes VII, B. Lewin, OxfordUniversity Press.
 References:
y
 
Introduction to Protein Structure, C. Branden and J Tooze, Garland Publishing Company.
y
 
Protein Structure, M. Perutz, OxfordUniversity Press.
y
 
Proteins (Structures and Molecular Properties), T.E. Creighton, W.H. Freeman and Company.
y
 
Database Annotation in Molecular Biology, Arthur M. Lesk.
y
 
From Genes to Clones, E.L. Winnacker.
y
 
Genes & Genomes, M.S. Paul Berg.
y
 
Structure and Machanism in Protein Science, Alan Fersht.

You're Reading a Free Preview

Download
/*********** DO NOT ALTER ANYTHING BELOW THIS LINE ! ************/ var s_code=s.t();if(s_code)document.write(s_code)//-->