Slide lab 4,5,6&7

Raed Al-Abbade & tahni abu alteen

 Lab 4

**Cross section**

Dentinal tubules Predentine

pulp ODONTOBLASTS
Dentine
 PDL
Granular layer
layer of Tome'
ome's

Radicular circumpulpal
dentine

Cementum
Alveolar bone
(structurless
structurless layer)hyaline
layer)hyaline layer

note : this picture show the different layers of the root

note : Granular layer of tomes lies directly below the hyaline layer
 Lab 5

Dentine
Predentine

1 = odontoblastic layer
2 = free-cell zone of Weil
3 = cell-rich zone
4 = pulp core
Demo. :

**** Subodontoblastic zone appear after tooth eruption &

composed of 2 layers :

1- free-cell zone of weil: (in crown only):

result from migration of central cells more than
local division of cells .
features:
anuclear ( not contain cells nuclei )
but contain processes of fibroblasts &
odontoblasts , axons and capillaries .
2- cell-rich zone :
contain capillaries (Subodontoblastic capillary
plexus ), axons (Subodontoblastic neural plexus =
plexus of Raschkow ).

2 1 Predentine
Predentine Dentine
Blood vessels
1 = Supraodontoblastic layer
2 = odontoblastic layer

Note : Supraodontoblastic layer : is potential (i.e.: it

is not found in vital but appear after histological
preparations as a result from shrinkage of the soft
pulp away from the dentine ) space between
odontoblastic layer & Predentine.
**It contains 2 things :
1- unsheathed axons .
2- dentritic antigen presenting cells .
**it is the 1st level at which the pulp can detect
external stimuli.

Note : odontoblastic layer is the effector system of

the pulp & all other cells of the pulp core serve to
protect and support it.
 Lab 6
Rabitt tooth --------------enamel organ----------bell stage

Dentine
Dentine

Dental
Dental papilla
ERS cells (of Hertwig)

EEE
 Demo:

*** ERS cells (of Hertwig):

Formed at late bell stage when amelogenesis & dentinogenesis
are well advanced .
Derived from the cervical loop of the enamel organ
Consists of 2 layers IEE +EEE only
Not contain stellate reticulum(S.R) or stratum
intermedium (S.I) cells.
Function : 1- map out the shape of the root &
2- shelves extend from its margins divide the primary
apical foramen into secondary apical foramina so
determine the number of roots in the tooth .
 Enamel
Odontoblasts
Dental pulp
Dentine

Ameloblasts Epithelial diaphragm

Proliferative zone of D.Follicle

Proliferative zone of D. Pulp

**Note : Epithelial root diaphragm extends apically & it is the

only region of the ERS(epithelial root sheath) , that it is
continues all the time .
 Lab 7

Collagen bundles of PDL

dentine
dentine

Acellular
cementum
PDL

Alveolar bone
Blood vessels
Cementoblasts

Fibroblasts
Epithelial rests of
malassaez
Note : Epithelial rests of malassaz results from disintegration of
the ERS above the root diaphragm.;;;;; it can be distigushed
from adjacent fibroblasts by the close packing of their cuboidal
cells and their tendency to stain more deeply.

Cellular
cementum
( Contain
Dentine cementocytes ) PDL

Osteoblasts
Cementocytes
Osteocytes

blood vessel osteoclasts (in Howship's lacunae)

**Note : Osteocytes don’t exhibit obvious canalculi in routine
demineralized sections (decalcified sections)

***Note : osteoblasts are specialized fibroblast-like cells of

mesenchymal origin.

Cementoclasts

Cellular
cementum
( Contain
cementocy
tes )

Dentine
entine