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séquençage d'ADN

séquençage d'ADN

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Published by Fati
un résumé de deux méthodes de séquençage
un résumé de deux méthodes de séquençage

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Published by: Fati on Sep 05, 2008
Copyright:Attribution Non-commercial

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05/09/2014

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 BIN1001, H2005, Hervé Philippe, U1
Méthodes de séquençaged’ADN
Rappel : ADN, Clonage, Électrophorèse
Méthode chimique de Maxam et Gilbert
Méthode enzymatique de Sanger
Détails des techniques utilisées au début desannées 1990
Séquençage de grands fragments : marche sur lechromosome, sous-clonage, etc.
Automatisation
Méthode Shotgun
 
 BIN1001, H2005, Hervé Philippe, U2
Rappel sur l’ADN
http://www.dgpc.ulaval.ca/bio90192/chap4/notesadn/notesadn1.htm
Base azotéeDésoxyribosePhosphate
 
 BIN1001, H2005, Hervé Philippe, U3
Enzymes de restriction
http://www.dil.univ-mrs.fr/~vancan/optionBio1/documents/1ercours2003_4.pdf 

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