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Oral histology
Dental pulp :
Crown -> coronal pulp - pulp chamber
Normally we have more than one root ,more than one root canal ,in
most of the cases each root has one root canal ,but in some of
posterior teeth we may see two root canals in one root ..e.g. in (60-
70) % there are two canals in the mesial root of mandibular first molar
,although it's one root ,but we see two canals. so this mean the
number of root canals isn't necessarily equal to the number of roots.
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Pulp:
is a specialized connective tissue ; it's connective tissue, but it's
specialized for reasons :
The rest of dental pulp is just simple connective tissue y3ne the core
of the pulp is simple acts as a support for the peripheral component .
the most important part of pulp are the peripheral component & the
central portion acts to support the peripheral component .
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What do we have in the core of pulp?
We have blood vessels & nerves that enter & leave through an apical
foramen (always we have in the apex of the root a small foramen in
which the nerves & vessels enter & leave ).
also the central portion of pulp contain cells , collagen and ground
material to support the peripheral component.
Accessory canal :
We have in addition to the main root canal that exists in the root we
may have accessory canal .in general we have one root canal
(radicular pulp) and accessory canal which exists on the sides of
pulp , acts in opened the inner of the pulp on the periodontal
ligament.
They are called lateral root canal ;when they made a communication
between the pulp & periodontal ligament ,but when they present at
the furcation area , they communicate between pulp chamber&
periodontal ligaments.. We call it furcation canal ( في مناطق انفصال
)الجذر.
fibers
non fibers matrix which is the semi fluid gel
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In histology --any connective tissue based on "3" substances: cells
,fibers & ground substances . also pulp as connective tissue contains :
cells ,fibers & semi fluid gel as the ground substance .
Matrix is more plentiful than cells; y3ne the amount of fibers & non
fibers matrix is more plentiful; much more than cells.
Function of matrix :
1-Forms a scaffold that stabilize the structure of the tissue: all the
component must be contiguous to each other. The surrounding
medium which act in protection the shape of pulp are semi fluid gel &
fibers. Without fibers, it will be easily collapsing of the odontobalst
cells & if that happened dentine will be separated from the process &
lose its function.
The fact that we have a scaffold that based on extra cellular matrix
that stabilize the structure of the tissue .
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Fibers :
Collagen
Type I-- is the most predominant one ( 56% ) ..fibers are thin &
scattered in young teeth they have a regular arrangement near
predentine fibers arranges parallel to predentine surface.
- We have also type III (40%) and type V & type VI in a small
amount.
fibrilln
Seconed type of fibers .these are large glycoprotein associated with
elastic fibers in other tissues. But in the pulp they are glycoprotein
only. elastic fibers are absent in the dental pulp, just fibrillin .
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Easy movement of water-soluble molecules
متل كأنه قاعدين بقاعة وفي خالياعلى االطراف ولحتى ما تسقط الخاليا بحاجة لضغط كبيرعشان
2 باتجاه االطراف2يدفعها
*Fibroblast
*Defence cells
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*Undifferentiated cells..they are important ,because when tooth is
subjected to injury, we will have a number of odontoblast killed by this
injury, so we need a new population of cells. We get them back from
undifferentiated cells.
Odontoblast :
Odontoblast are responsible in forming of dentine .they survive for as
long as the tooth is vital .
تفقد عند خلع السن او نزع العصب, طالما السن حي طالما هذه الخاليا موجودة
Polarized : means that the nucleus isn't located in the center of the
cell , nucleus tends to be away from dentine, polarized columnar cells
with process extending within a tubule.
In root region , cells are more cuboidal , in the root the inner surface
of dentine is smaller so the odontoblast cells are shorter . There aren't
enough space to get its suitable length .
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Membrane-like properties. They are cells bounded closely to
each other..so we could consider odontoblastic layer a
membrane separate outer medium from the pulp.
Act as a barrier to protect the dental pulp from outside irritants :
بعض2يعني متل كأنه احضرنا مجموعة كبيرة من الناس و صفيناهم على الحيط بجانب بعضهم
يجدون امامهم حوائط بشرية تمنعهم و ما رح يقدروا2بالتالي الناس الذين من الخارج سوف
يقتحموا.
Fibroblast :
They are the second type of cells present at the pulp , they are linked by
adherence type junction & gap junction . these cells are stellate cells
( star-like ) they have many extensions ; each cell's extensions are linked
to the extensions of the other cell . they undergo cell division and they
have the following functions :
They produce the fibers of the dental pulp and the ground
substance and participate in their degradation ( and are
responsible for forming a new one as well ) .
They may produce bone-like mineralized tissue as a response to
pulpal injury . sometimes when the pulp faces a chronic stimulus ,
instead of the repair mechanism for the tissue it becomes a pulp
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stone ( calcified pulp tissue ) and the fibroblast are the cells that
forms this stone in most of the cases.
They produce the Growth Factors ( GFs) & cytokines .
Defence cells :
Blood vessels :
*So nerves can pass through dentinal tubules but blood vessels
can't . ( dr said ensures us to memorize this) .
Nerves :
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Nerves run along side the blood vessels In the center of the
pulp . they branch profusely In the odontoblastic &
subodontoblastic regions .
In the crown , the subodontoblastic plexus is called plexus of
Raschkow .
This is evident only after eruption ; we can see this
plexus only after the eruption of the tooth .
Some branches reach between odontoblast and
predentine while others continue and join the
processes within the tubules .
May be a site of sensory activation as evident because
axons lack Schwann cell covering ; these axons when
passing through dentine it becomes unmylinated so it
can be directly stimulated and we called this the direct
stimulation theory .
Nerve endings :
Regions :
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These layers are : the supraodontoblastic layer , the
odontoblastic layer , the subodontoblastic zone and the
central region respectively .
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Meanwhile in the vital pulp , there is no such space
because there is always good amount of pressure pushing
the odontoblast so it don't move from its space.
dental odontoblasts.
o we have the zone of weil & cell rich zone ; you can see that
directly below the odontoblasts there is a layer that is free of cells
( no cells there ) so its called the Cell-free zone of weil . and below
this layer there is a layer that has a lot of cells and so its called the
Cell-rich zone .
o in cell free zone of weil : we don't see any cells there , only axons
& cell processes of fibroblasts & other cells , and this layer is
usually absent in radicular pulp .
( and we all know that in light microscope we only see the nuclei of cells
and not the cell walls , so when there are no nuclei or no cell bodies we
see a space , this space might have other structures like axons ,
processes , etc )
o In cell rich zone : this layer has lots of cells and appears in contrast
to the cell free zone ( )تظهر بالمقارنة مع المنطقة الخالية من الخاليا,
capillaries and nerve plexuses that contain the cell bodies of
schwann cells , endothelial cells , etc.
Now in central region : we see the bulk of the dental pulp , the
cental neurovascular core which the core that has the bundle of
the major blood vessels and the major nerves , we see also
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fibroblast , defence cells and undifferentiated cells and
collagenous matrix and ground substance . also we see thick blood
vessels in this area as you can see in the picture above .
What happens to your dental pulp when you get advanced in your
age ?
First of all , the pulp becomes smaller because of the addition of
secondary dentine , and in cases of tertiary dentine the pulp gets
even much smaller , this leads to the reduction in the vascular ,
neural and cellular contents , increase in the fibrous matrix
( because fibroblasts have been producing fibers and ground
substances for a long time and so the content of the extracellular
fibers will be greater than before while the number of cells will
decrease ) .
Also we see some degree of mineralization ( يمكن حدوث تكلس داخل
)اللبthis will be in the form of either a pulp stones or huge
calcification called snow storm calcification .
o Pulp stones appears as spheres or globules , While in snow storm
calcification the whole pulp looks calcified ( ) و كأنه تم رشه بمادة مكلسة
so the calcification in this type is spread evenly inside the pulp.
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o Pulp stones can be single or groups , it can be free inside the pulp
or attached to the dentine , and most importantly it can be true
or false , true when it is dentine-like ; looking exactly like dentine
having dentinal tubules , peritubular dentine and intertubular
dentine , etc . and true pulp stone is formed by odontoblasts .
However , False pulp stones is Bone-like and is formed by
hydroblasts .
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DID You know that : pulp stones are extremely
common , occurring in as many as 90 percent of people between
the age of 50 and 70 , it is not known precisely what causes pulp
stones but they are really painful and they require surgery in most
of the cases!
وأعطاك فهما، و منعك إثما، ومنحك علما, مازادك حلما: أفضل االيام.
DONE BY :
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