AS OCR Biology Revision PackUNIT f211 Cells, exchange, transportModule 1 CellsCell Structure
1. State the resolution and magnification that can be achieved by a light microscope, a transmission electron microscopeand a scanning electron microscope.
ight Microscope TEM SEMMaximumResolution
0.2 micrometres 0.0001 micrometres 0.005 micrometres
X 1500 Over x 1,000,000 Under x 1, 000, 000
2. Explain the difference between magnification and resolution
How much bigger the image is than the specimen.Magnification = Length of Image / Length of specimen
How well a microscope distinguishes between two points that are closetogether.
3. Explain the need for staining samples for use in light and electron microscopy
In Light microscopes and TEM¶s the beam of lights/electrons pass through the object,and there is an image produced as some parts of the specimen absorb morelight/electrons than others, but sometimes the specimen is transparent so it will lookwhite because light/electrons pass through so the object is stained
Dye- usuallymethylene blue/eosin Specimen dipped in metal like lead, themetal ions scatter electrons to contrast.
4. Calculate the linear magnification of an image
ength of Image /
ength of specimen
5. Outline the functions of the structures.