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ABI Prism 7000 Sequence Detection System User Guide_2

ABI Prism 7000 Sequence Detection System User Guide_2

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Published by: Aqwin Polosoro on May 27, 2011
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07/20/2014

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Getting Started Guide
 Applied Biosystems
Relative Quantification
 ABIP
RISM®
7000 Sequence Detection System
IntroductionPerformingReverseTranscriptionDesigning anRQ ExperimentGeneratingData from RQPlatesPerforming anRQ Study
Primer Extended on mRNA
5
3
ReversePrimer5
Synthesis of1stcDNA strand
 
cDNA
 
Oligo d(T)orrandomhexamer
3
5
 
cDNA
 
   S   A   M   P   L   E   D   O   C   U   M   E   N   T
   S  e  p   t  e  m   b  e  r   2   6 ,   2   0   0   3   3  :   1   7  p  m ,   G   S   G_   F  r  o  n   t   C  o  v  e  r .   f  m
ii
Relative Quantification Getting Started Guide for 7000 v1.1
©Copyright 2003, Applied Biosystems. All rights reserved.For Research Use Only. Not for use in diagnostic procedures.
 NOTICE TO PURCHASER:PLEASE REFER TO THE ABIP
RISM®
7000 SEQUENCE DETECTION SYSTEM USER’S MANUAL FOR LIMITED LABEL LICENSE OR DISCLAIMER INFORMATION.
Trademarks
Applied Biosystems, MicroAmp, Primer Express, and VIC are registered trademarks of Applera Corporation or itssubsidiaries in the US and/or certain other countries.AB (Design), ABIP
RISM
, Applera, Assays-by-Design, Assays-on-Demand, FAM, and MultiScribe are trademarks of Applera Corporation or its subsidiaries in the US and/or certain other countries.AmpErase, AmpliTaq Gold, and TaqMan are registered trademarks of Roche Molecular Systems, Inc.SYBR is a registered trademark of Molecular Probes, Inc.Microsoft and Windows are registered trademarks of Microsoft Corporation.All other trademarks are the sole property of their respective owners.Part Number 4346727 Rev. A9/2003
 
PrimerExtended on mRNA
5
3
ReversePrimer5
Synthesis of1stcDNA strand
 
cDNA
 
Oligo d(T)orrandomhexamer
3
5
 
cDNA
Relative Quantification Getting Started Guide for 7000 v1.1
iii
23415
Introduction
Introduces concepts related to relative quantification (RQ) experiments using the ABIP
RISM
®
7000Sequence Detection System and provides an overview of the RQ Study workflow.
Designing an RQ Experiment
Describes the required components of an RQ experiment and describes the set up of a sample RQexperiment. Also provides a list of the materials and equipment required for RQ studies.
Performing Reverse Transcription
Outlines the preferred methodology for reverse transcribing total RNA to cDNA and provides guidelines for RNA quality and starting amounts of total RNA.
Generating Data from RQ Plates
Explains how data generated during the PCR process is captured in RQ Plate documents and provides information about analyzing RQ Plate data.
Performing an RQ Study
Explains how to analyze data from one or more RQ Plate documents and to save the results in an RQStudy document.

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