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Measuring the Rate of Oxygen Uptake

Measuring the Rate of Oxygen Uptake

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Published by Sachitra Wijethunga

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Published by: Sachitra Wijethunga on Jun 04, 2011
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05/04/2013

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Edexcel practical materials created by Salters-Nueld Advanced Biology, ©University o York Science Education Group.
Practical 7.1
Measuring the rate of oxygen uptake
Respirometers
Respirometers range rom relatively simple pieces o equipment used in school sciencelabs with seeds or invertebrates, to elaborate devices the size o a room used to measurerespiration rates in humans living near-normal lives over a period o several days. In thispractical you will be using a very simple respirometer, while considering the advantages o some o the slightly more complex ones.
Procedure
1
Assemble the apparatus as shown in the diagram below.
 A simple respirometer 
2
Place 5 g o maggots or peas into the test tube and replace the bung.
•Todemonstratetheuptakeofoxygeninrespiration.•Tomeasuretherateatwhichanorganismrespires.
Purpose
Wear eye protection when handling soda lime.Soda lime is corrosive. Do not handle directly:use a spatula.
Safety
scale1
 
cm
3
pipette or glass tubesyringeglass tubingsmall organismsgauzesoda limethree-way tapcoloured liquid
Sodalime•Colouredliquid•Droppingpipette•PermanentOHTmarkerpen•Solvent(toremovethemarker)•CottonwoolStopclock•EyeprotectionYouwillneed:•Respirometer(seediagrambelow)•5gofanactivelyrespiringorganism
Student
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