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The Effect of Temperature on the Hatching Success Of

The Effect of Temperature on the Hatching Success Of

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Published by Sachitra Wijethunga

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Published by: Sachitra Wijethunga on Jun 04, 2011
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08/04/2012

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Edexcel practical materials created by Salters-Nufeld Advanced Biology, ©University o York Science Education Group.
Student
1 of 2
Practical 5.2
The effect of temperature on the hatching success ofbrine shrimps
Brine shrimps
Brine shrimps are small, saltwater crustaceans; the adults are about 8 mm in length. Theyare relatively easy to keep in the laboratory and will produce dormant egg cysts that hatch toproduce young shrimp larvae.
Drawings to show features of brine shrimps
Procedure
1
Decide on a range o temperatures rom 5 °C to 35 °C to be tested.
2
Place 2 g o sea salt into a 100 cm
3
beaker.
3
Add 100 cm
3
o de-chlorinated water and stir until the salt completely dissolves.
4
Label the beaker with sea salt and the temperature at which it will be incubated.
5
Place a tiny pinch o egg cysts onto a large sheet o white paper.
•Toinvestigatetheeffectoftemperatureonthehatchingsuccessofbrineshrimps.•Todevelopcertainexperimentalskills,namelyconsideringtheethicalissuesarisingfromtheuseoflivingorganisms,presentingresults,producingreliableresults,identifyingtrendsindataanddrawingvalidconclusions.
Purpose
1
 
mmeggssecondantennafemale(brownish red)firstantennamale(blue/green)
•Stirringrod•Magnifyingglass•Pairofforceps•Fineglasspipette•Brightlight•Accesstorefrigerator•SheetofA4whitepaper•Sheetofgraphpaper3cm
3
4cmYouwillneed:•Brineshrimpeggcysts•2gseasaltforeachtreatment100cm
3
de-chlorinatedwaterforeachtreatment40cm
3
beakerofsaltwater100cm
3
beakers(oneforeachtemperaturetobetested)•Waterbathsorincubators(oneforeachtemperaturetobeinvestigated)

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