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Microscope & Lens Cleaning

Microscope & Lens Cleaning

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Published by EMQueen

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Categories:Types, Research, Science
Published by: EMQueen on Jul 03, 2011
Copyright:Attribution Non-commercial

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06/20/2014

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The Clean Microscope
Recognizing dirtand removing it correctly
Principles
 
Microscopy from Carl Zeiss
We make it visible.
 
Clean microscope optics are a prerequisite for suc-cessful microscopy and perfect images.Over the years a variety of cleaning procedureshave been recommended. Many users remain un-sure as to which of these will yield the best results.The choice of the best cleaning method depends onthe nature of the optical surface and the type ofdirt to be removed.Please contact the local Carl Zeiss representativefor questions regarding maintenance and service.
Where to find what?
PageAcetone . . . . . . . . . . . . . . . . . . . . . . . . .5, 9Anisole . . . . . . . . . . . . . . . . . . . . . . . . . . . .4Blue WINDEX . . . . . . . . . . . . . . . . . . . . . . .5Camera adaptor . . . . . . . . . . . . . . . . . . . . .5Cameras . . . . . . . . . . . . . . . . . . . . . . . . . . .5Chloroform . . . . . . . . . . . . . . . . . . . . . . . . .5Cleaning motion . . . . . . . . . . . . . . . . . . . . .9Cleaning Solution L . . . . . . . . . . . . . .6, 9,12Coatings . . . . . . . . . . . . . . . . . . . . . . . . . . .5Cotton . . . . . . . . . . . . . . . . . . . . . .6, 7, 8,12Cover slips . . . . . . . . . . . . . . . . . . . . .4, 6, 7Diethylether . . . . . . . . . . . . . . . . . . . . . . . .9Dirt – greasy, loose, oily, water-soluble . . . . .8Dirt location . . . . . . . . . . . . . . . . . . . . . . . .3Dirt on the camera . . . . . . . . . . . . . . . . . . .3Dirty front lens . . . . . . . . . . . . . . . . . . . .2, 3Dry objective with correction collar . . . . . . .4Dust blower . . . . . . . . . . . . . . . . . . . . . .6, 8Dust . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10Ethanol . . . . . . . . . . . . . . . . . . . . . . . . . . . .9Fluorescence filter sets . . . . . . . . . . . . . . . . .5Fungus . . . . . . . . . . . . . . . . . . . . . . . . . . .10IMMERSOL . . . . . . . . . . . . . . . . . . . . . . .2, 4JENA-Microscopes 250 CF . . . . . . . . . . . . .11Kleenex . . . . . . . . . . . . . . . . . . . . . . . . . . .6LD-Dry objective . . . . . . . . . . . . . . . . . . . . .2MIKROVAL-Microscopes . . . . . . . . . . . . . . .11Optical surfaces – concave or convex . . . .5, 6Optical surfaces – flat . . . . . . . . . . . . . . .5, 6Painted surface . . . . . . . . . . . . . . . . . . . . .10Petroleum ether . . . . . . . . . . . . . . . . .6, 9,12Polyester swab . . . . . . . . . . . . . . . . . . .6, 7, 8Polystyrene stick . . . . . . . . . . . . . . . . . . . .11SIDOLIN . . . . . . . . . . . . . . . . . . . . . . . . . . .5Solvents . . . . . . . . . . . . . . . . . . . . . . . . .6, 8SPARKLE . . . . . . . . . . . . . . . . . . . . . . . . . . .5Spherical aberration . . . . . . . . . . . . . . . . . .4STANDARD-Microscopes . . . . . . . . . . . . . . .11Swabs . . . . . . . . . . . . . . . . . . . . . .6, 7, 8,12WHATMAN paper . . . . . . . . . . . . . . . .6, 7,12Xylene . . . . . . . . . . . . . . . . . . . . . . . . . . . .9
 
The Effect of Dirton the Image
2
The closer dirt is to the object or to the camerasensor, the greater its effect on the visual orphotographed image. The critical areas are thefollowing:1. The external surface of the front lens of theobjective2. The surface of the camera sensor and itsprotective glass cover3. Both surfaces of the cover slip4. The surface of the microscope slide5. The surface of the camera adapter optics6. Surface of the upper lens of the condenser7. The outer and inner surfaces of the eyelensof the eyepiece as well as the upper surfaceof the graticule8. The outer surface of the protective glass cover-ing the opening through which light exits9. Other glass surfaces in the light path e.g. bulbsof halogen- or high pressure lights, fluores-
cence filters and beam splitters, collector lenses,
contrast and heat filters.Some optical surfaces are more sensitive to dirtthan others. The front lens of the objective is par-ticularly critical and is therefore discussed ingreater detail below:For any dry objective, the smaller the free workingdistance and the smaller the surface area of theconcave front lens, the greater the danger of soil-ing the front lens with embedding media, immer-sion liquids or dust.Examples of such objectives are the EC Plan-Neo-fluar 40x/0.75, EC Plan-Neofluar 63x/0.95 Korr,Achroplan 63x/0.80, 63x/0.95 o.D., Fluar 20x/ 0.75, Planapo 20x/0.80, Planapo 40x/0.95 Korr, alldry objectives of the type Epiplan and EC Epiplan-Neofluar as well as EC Epiplan-Apo-Objectives withmagnifications of 20x, 50x and 100x.When working with inverted microscopes, thefront lens of every objective will be more exposedto dust than that in an upright microscope; all LDdry objectives with magnifications of 32x, 40x and63x need to be regularly checked.The front lens of an immersion objective should becleaned to remove residue both after use and addi-tionally, before applying fresh immersion liquid.The mixing of different immersion media, as wellas different lots of the same medium e.g. theimmersion oil IMMERSOL F™, can result in blurredimages.The cameras are always to be handled with theutmost care and protected from dirt using all avail-able methods.
Before every critical use, check the front lensof the objective for dirt.
Title:Toad,liver,stained with Azan.Planapo 63/1.4.Bright field 

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