Miss Phratchaya Seeladlao. ID: 5231105037
5. Compare a contrast the protocols used the extract DNA, RNA andprotein
- The identification and validation of molecular markers DNA, RNA and microRNA based on biomarkers and when compared the DNA/RNA obtained with theoptimized protocol and they include variable cell lysis times and differentchemistries for the extraction of RNA and DNA in addition, deparaffinization inthe RNA/DNA extraction protocol by Hurt et al. Furthermore, to process manysamples in parallel, a miniaturization of the protocol was required. However,when we applied the protocol with the modifications, the RNA obtained wasfrequently degraded. Since RNA is often preserved as ethanol precipitate, weexplored the idea of adding RNA protecting substances such as ethanol,isopropanol or the denaturing solution used by Hurt et al. already beforebreaking up the cells by means of a bead beating step. Agarose gelelectrophoresis are differ the concentration base on size of biomolecule(DNA/RNA extraction from FFPE tissue samples),- In Proteins were extracted using TCA in acetone (TCA-acetone), phenol, ormulti-detergents in a chaotrope solution, extracted proteins were solubilized in amultiple chaotrope solution and examined using 1-D and 2-D electrophoresis andcompared directly using 2-D Difference Gel Electrophoresis (2-D DIGE). Massspectrometry was used to identify proteins from each extraction type (Acomparison of Protein Extraction Methods Suitable for Gel-Based ProteomicStudies of Aphid Proteins).
6. Compare & contrast the protocols used to extract genomic DNA frombacteria, fungi, plant and animal cells
The goal of genomic DNA isolation depends on what the applications of the DNA after isolation. Purity, source, quantity and quality of DNA are all issuesthat need to be addressed prior to genomic DNA extraction. A whole host of different methods, technologies and kits are available now to researchers toisolate genomic DNA from cells.- Quantity of DNA needed- Molecular weight and size of DNA- Purity of DNA required- Downstream applications of DNA- Time available- Ease of DNA extraction technique or method- Expense or money available