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Home Work 3 LAb Microbial Genetic

Home Work 3 LAb Microbial Genetic

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Published by Fern S. Phratchaya

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Published by: Fern S. Phratchaya on Jul 24, 2011
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01/09/2014

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Miss Phratchaya Seeladlao. ID: 5231105037
Home work 3 (Lab) MicrobialGenetic
1. Describe the usefulness of gene cloning also give an example of youranswer
- Cloning is the producing many identical copies of the same recombinant mustbe replicated many times to provide material for analysis, sequencing, etc. Theterm 'cloning' have entered popular vocabulary of many people. The issues oncloning are getting controversial and growing more complicated the word "clone"comes from the Greek "klwn" which means similar.For example cloning usefulnessNEW YORK — It may be futile to try producing stem cells by putting human DNAinto cow or rabbit eggs and making hybrid cloned embryos, a strategy thattriggered controversy recently in Britain.Cloning gives parents the opportunity to choose what characteristics they wanttheir children to have. For instance, the parents want their child to have AlbertEinstein’s IQ. The extraordinary athleticism of Michael Jordan.Horticultural: The term clone is used in horticulture to refer to descendants of asingle plant which were produced by vegetative reproduction or apomixis. Manyhorticultural plant cultivars are clones, having been derived from a singleindividual, multiplied by some process other than sexual reproduction. As anexample, some European cultivars of grapes represent clones that have beenpropagated for over two millennia. Other examples are potato and banana. The hormone insulin or growth hormones, the first hormone that is produced bythis method is that insulin can be synthesized in bacteria E. coli.
3. Apart form bacterial host is there any other host cell that we canuse? If so, what are they? (provide the paper conclude abstract page) tosupport your answer
Definition noun, plural: host cells-A cell that harbors foreign molecules, viruses, or microorganisms. For example,a cell being host to a virus.- A cell that has been introduced with DNA (or RNA), such as a bacterial cellacting as a host cell for the DNA isolated from a bacteriophage.Other host cell (Cell Culture), (Embryonated egg) and (Experimental animal)
 
Miss Phratchaya Seeladlao. ID: 5231105037Iv model in eukaryotic cell used yeast, Yeast is a popular host as it is a eukaryotewith similar synthetic machinery to that of the native human source cells of many proteins of interest, while also being quick, easy and cheap to grow andprocess.
Understanding the yeast host cell response to recombinant membrane proteinproduction.
Source
School of Life and Health Sciences, Aston University, Birmingham, U.K.
Abstract
Membrane proteins are drug targets for a wide range of diseases. Having access to appropriate samples for further research underpins the pharmaceutical industry's strategy for developing new drugs. This is typicallyachieved by synthesizing a protein of interest in host cells that can be cultured on a large scale, allowing theisolation of the pure protein in quantities much higher than those found in the protein's native source. Yeast is apopular host as it is a eukaryote with similar synthetic machinery to that of the native human source cells of manyproteins of interest, while also being quick, easy and cheap to grow and process. Even in these cells, theproduction of human membrane proteins can be plagued by low functional yields; we wish to understand why.We have identified molecular mechanisms and culture parameters underpinning high yields and haveconsolidated our findings to engineer improved yeast host strains. By relieving the bottlenecks to recombinantmembrane protein production in yeast, we aim to contribute to the drug discovery pipeline, while providing insightinto translational processes.
 
Miss Phratchaya Seeladlao. ID: 5231105037
5. Compare a contrast the protocols used the extract DNA, RNA andprotein
- The identification and validation of molecular markers DNA, RNA and microRNA based on biomarkers and when compared the DNA/RNA obtained with theoptimized protocol and they include variable cell lysis times and differentchemistries for the extraction of RNA and DNA in addition, deparaffinization inthe RNA/DNA extraction protocol by Hurt et al. Furthermore, to process manysamples in parallel, a miniaturization of the protocol was required. However,when we applied the protocol with the modifications, the RNA obtained wasfrequently degraded. Since RNA is often preserved as ethanol precipitate, weexplored the idea of adding RNA protecting substances such as ethanol,isopropanol or the denaturing solution used by Hurt et al. already beforebreaking up the cells by means of a bead beating step. Agarose gelelectrophoresis are differ the concentration base on size of biomolecule(DNA/RNA extraction from FFPE tissue samples),- In Proteins were extracted using TCA in acetone (TCA-acetone), phenol, ormulti-detergents in a chaotrope solution, extracted proteins were solubilized in amultiple chaotrope solution and examined using 1-D and 2-D electrophoresis andcompared directly using 2-D Difference Gel Electrophoresis (2-D DIGE). Massspectrometry was used to identify proteins from each extraction type (Acomparison of Protein Extraction Methods Suitable for Gel-Based ProteomicStudies of Aphid Proteins).
6. Compare & contrast the protocols used to extract genomic DNA frombacteria, fungi, plant and animal cells
The goal of genomic DNA isolation depends on what the applications of the DNA after isolation. Purity, source, quantity and quality of DNA are all issuesthat need to be addressed prior to genomic DNA extraction. A whole host of different methods, technologies and kits are available now to researchers toisolate genomic DNA from cells.- Quantity of DNA needed- Molecular weight and size of DNA- Purity of DNA required- Downstream applications of DNA- Time available- Ease of DNA extraction technique or method- Expense or money available

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