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rr322302-analytical-techniques-in-biotechnology

rr322302-analytical-techniques-in-biotechnology

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Published by: SRINIVASA RAO GANTA on Sep 18, 2008
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Code No: RR322302
Set No. 1
III B.Tech Supplimentary Examinations, Aug/Sep 2008
ANALYTICAL TECHNIQUES IN BIOTECHNOLOGY
(Bio-Technology)
Time: 3 hours
Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
\u22c6 \u22c6 \u22c6 \u22c6 \u22c6
1. In brief compare the di\ufb00erent types of light microscopes.

[16] 2. Write in detail the description of a typical microbiological light microscope. [16] 3. (a) Distinguish between Nucleotides and Nucleosides

(b) Deoxyribose and ribose
[8+8]
4. What are the factors a\ufb00ecting electrophoretic mobility of any molecule?
[16]
5. Explain the principle in Southern Hybridization.
[16]
6. Explain antisera production in detail.
[16]
7. Which is the most commonly used Radio Isotope for radio labeling antibodies?
Describe it.
[16]
8. What is the di\ufb00erence between BOD and COD?
[16]
\u22c6 \u22c6 \u22c6 \u22c6 \u22c6
1 of 1
Code No: RR322302
Set No. 2
III B.Tech Supplimentary Examinations, Aug/Sep 2008
ANALYTICAL TECHNIQUES IN BIOTECHNOLOGY
(Bio-Technology)
Time: 3 hours
Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
\u22c6 \u22c6 \u22c6 \u22c6 \u22c6
1. Explain the following terms associated with Fluorescent Microscope.
(a) Barrier \ufb01lter

(b) Heat \ufb01lter
(c) Fluorochromes
(d) Exciter \ufb01lter.

[4+4+4+4]

2. List out the prerequisites for viewing any biological specimen under TEM. [16] 3. What are Nucleotides? At what wavelength can they be absorbed and why? [16] 4. What is the role of SDS in SDS\u2212 PAGE?

[16]
5. Write short notes on
(a) Restriction Enzymes
(b) Nitrocellulose \ufb01lters.
[8+8]
6. How is PCR used to sequence DNA?
[16]
7. What is meant by Immunoblotting? What is it used for?
[16]
8. How is gaseous Oxygen concentrations measured?
[16]
\u22c6 \u22c6 \u22c6 \u22c6 \u22c6
1 of 1
Code No: RR322302
Set No. 3
III B.Tech Supplimentary Examinations, Aug/Sep 2008
ANALYTICAL TECHNIQUES IN BIOTECHNOLOGY
(Bio-Technology)
Time: 3 hours
Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
\u22c6 \u22c6 \u22c6 \u22c6 \u22c6
1. Describe a Dark Field Microscope.
[16]
2. Describe the construction of a TEM.
[16]
3. How are proteins detected biochemically in any sample?
[16]
4. What are the di\ufb00erences between horizontal and vertical Electrophoresis units?[16]
5. Discuss the importance of Elution time; volume and Capacity factor in Chromatog-
raphy.
[16]
6. Explain Maxam and Gilbert method of DNA sequencing.
[16]
7. Di\ufb00erentiate between single and double Electroimmunodi\ufb00usion.
[16]
8. What is meant by the term \u201cBiomass\u201d? Explain its relevance to cell growth. [16]
\u22c6 \u22c6 \u22c6 \u22c6 \u22c6
1 of 1

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