Write about different types of centrifuges and how they are scaled up. Compare the advantages disadvantages centrifugation with microfiltration.
10g of BSA is eluted from an 80 litre Sephadex column which has a void fraction of 0.4. The concentration in the column peaks after 470 litres are eluted and the maximum concentration is 1.8% of that originally in the column. Estimate
b) The number of stages in the column
c) The concentration profile in the column.
7.a) For disruption of baker\u2019s yeast, a homogenizer was found to give a yield of 49% when operated at 55 Mpa and 85% at 105 Mpa. Determine the number of passes required to obtain 85% disruption at 55 Mpa.
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