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Enterobactérie Final

Enterobactérie Final

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Published by ismail bd

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Published by: ismail bd on Sep 26, 2008
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02/17/2013

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Identification biochimiquedes espècesd’ Entérobactéries
 
Mme KAIDI A.
 
 
Le test est surtout utilisé pour différencier entre lesdifférents groupes genres et espèces des E
nterobacteriaceae 
 Le but de ce test est de mettre en évidence cinq caractères:
La fermentation duglucose,
La fermentation dusaccharose 
La fermentation dulactose
La production degaz
La production de sulfure d’hydrogène(H
2
S)à partir duthiosulfate dans un environnement acide
La fermentation du glucose seul montrera unviragede lacouleur au jaunedu culot
Celle du saccharose et du lactose se manifestera ar un
Recherche de l’utilisation du Glucose, saccharose etlactose, production de gaz et H
2
S sur milieu Triple SugarIron Agar (TSI)
 
 
Triple Sugar Iron Agar (TSI 1)
 
1
 
2
 
3
 
4
 
5
 
1 : Milieu non inoculé2 : G+, S+, L+, Gaz+,H
2
S- :
Klebsiella 
3 : G+, S+, L+, Gaz-,H2S-
Yersinia enteocolitica 
(18h)4 : G+, S+, L+, Gaz-,H2S- 
Yersinia enteocolitica 
 (48h)5 : G+, S+, L+, Gaz+,H
2
S+ 
Citrobacter 
6 : G+, S+, L+, H2S+,Gaz-
Erysipelothrix rhusiopathiae  
Fermente faiblement le
6
 

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