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Prokaryotic Genetics

Prokaryotic Genetics

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Published by Amrutha Basker

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Categories:Types, School Work
Published by: Amrutha Basker on Sep 17, 2011
Copyright:Attribution Non-commercial

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10/06/2014

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GENETIC RECOMBINATION INPROKARYOTES
 
Mechanisms of Genetic Exchange
Bacterial Conjugation 
 – 
Mechanism of transferring genetic information fromone bacterium to another, followed by recombination
with the recipient bacterium’s genetic material
 
Transformation
 – 
Uptake of DNA from the surrounding medium and
recombination into the recipient bacterium’s genetic
material
Transduction
 – 
Transfer of genetic material from one bacterium toanother via bacteriophage
 
Bacterial Conjugation
Discovered by Lederberg and Tatum (1946)
 – 
Two auxotroph strains (one was
met bio
and theother
thr leu thi
)
 – 
Culture together on complete medium
 – 
Subculture on minimal medium
Some cells were prototrophs (10
-7
)… and 2
-3 independentgene mutations (genetic exchange &recombination)unlikely to create revertants
One strain had provided genetic material to replacedefective genes in the other

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