Welcome to Scribd, the world's digital library. Read, publish, and share books and documents. See more
Download
Standard view
Full view
of .
Save to My Library
Look up keyword
Like this
18Activity
0 of .
Results for:
No results containing your search query
P. 1
Extração de Dna

Extração de Dna

Ratings: (0)|Views: 2,628 |Likes:
Published by leandrotavares07

More info:

Categories:Types, Research, Science
Published by: leandrotavares07 on Sep 29, 2011
Copyright:Attribution Non-commercial

Availability:

Read on Scribd mobile: iPhone, iPad and Android.
download as PDF, TXT or read online from Scribd
See more
See less

09/20/2013

pdf

text

original

 
Extração de DNA
•
identidade
dos seres ViVos
DNA: A receitA DAviDA e o seu cóDigo
vã: jan 23, 2010 11:33 AM
EXPERIMENTO
 
2
Extração de DNA
vã: jan 23, 2010 11:33 AM
1. rum
ea adad páa pbla a xaçã d DNA d man, q ambém pd  bíd p banana ma lzand maa d fál a.
2. o expm2.1 M: 
2  3 man (pd  bíd p ½ banana  ½ ma);
•
sa plá mm anpan;
•
Dn mal
•
Béq  p;
•
clh d afé;
•
Pa   fa m adaçã lméa;
•
Áll íl abl  áll íl dmé (>90g.L). (D  mand lad aé  mmn da a
•
lzaçã);
Gazeparaltrar;•
tb d na;
•
cl d d (al d znha);
•
Fnl;
•
Faa;
•
Baã d d  pal d mada;
•
Ppa Pa, na  na-a.
•
Figura 1: Materiais necessários
 
Extração de DNA
3
vã: jan 23, 2010 11:33 AM
2.2 Pcm:
sm q a adad ja alzada m mplmn à ala a b DNA.
Verique,inicialmente,quaissãoosconhecimentosdosalunossobreoconceitodeDNAcomoamolécularespon
-ál pla aaía d d   . Pn, p xmpl, qa anm pm DNA  qal é afnçã q a bâna m n  . Da m l nd  DNA é nnad na élla aa: DNA é  pp mm? s ná, lmb  ní d anzaçã d maal né (d mmaé a dpla hél d DNA) paa lmba q  DNA  nna aad à mléla d pína. ea nfma-çõ ã ú paa q  aln mpndam mlh ada apa d p d xaçã d DNA.É mpan d m  aln a qã da dmnõ: é píl nxa élla a lh n? e   núl?e  mm? e a dpla hél? i é fndamnal paa nã a n aln a xpaa qada d q aadad pmá q l alz a “dpla hél” d DNA.Dp d ad  pnpa n, dba   d abalh  xplq qal é  bj d xp-mn  d  pdmn.
2.2.1 Pcl expml
Preparo da Solução de “Lise” (ruptura das membranas celulares): 
Ma 6 mL d dn, 4 d Nacl (al d znha),  qaln a apxmadamn 4 lh had afé,  mpla m áa aé 60 mL. 
 AFigura 2: Preparo da solução de lise com (A) sal de cozinha, (B) detergente e (C) água.BC

Activity (18)

You've already reviewed this. Edit your review.
Edilane Xavier liked this
1 thousand reads
1 hundred reads
Tales Martins liked this
toycly liked this
Fátima Eusébio liked this
Erivelto Trygon liked this
Marcos Woitowitz liked this

You're Reading a Free Preview

Download
/*********** DO NOT ALTER ANYTHING BELOW THIS LINE ! ************/ var s_code=s.t();if(s_code)document.write(s_code)//-->