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Microtome Procedure

Microtome Procedure

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Published by Microtomist
The document lists step by step procedure of cutting sections on a microtome
The document lists step by step procedure of cutting sections on a microtome

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Published by: Microtomist on Oct 01, 2011
Copyright:Attribution Non-commercial

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08/23/2014

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1.Wind back the coarse feeding mechanism
and lock the hand wheel
2.Clamp cassette in position, orientating thetissue as desired3.Ensuring that the knife holder is a safedistance from the cassette, carefully place ablade into the holder and tighten securely
 
4.Loosen back lever of knife holder and
advance towards the cassette until there is a
0.2-0.5mm interval between blade edge andcassette surface
5.Tighten back the lever of knife holder
6. Unlock wheel and trim away surplus waxfrom tissue surface by advancing the coarse
feed mechanism before commencing each
turn of the wheel

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