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Para lab 9

Para lab 9

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Published by: api-3743217 on Oct 15, 2008
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04/02/2014

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PARASITOLOGY LABORATORY 9 \u2013 Protozoans
USTMED \u201907 Sec C \u2013 AsM; Photos provided by JV.N.
E. Histolytica Cyst

Mature cysts of E. histolytica have four nuclei while immature cysts contain one or two nuclei. The cysts range in size from 10 to 18 um and frequently have a diffuse glycogen vacuole, as in this case. Red chromatoid bodies with rounded ends are frequently found. The karyosomes may be slightly eccentrically located, and the peripheral chromatin is fine and evenly distributed.

E. Histolytica Trophozoites
Entamoeba histolytica.
Trophozoites. Feces. Trichrome
stain. The trophozoites ofE.
histolytica are round and measure

from 15 to 30 um in diameter. With the trichrome stain the cytoplasm stains green-magenta, and the nucleus has a central karyosome. The chromatin has a delicate appearance and is distributed

uniformly in the periphery along the nuclear membrane.
Entamoeba histolytica.

Trophozoites. Feces. Trichrome stain. This E. histolytica trophozoite shows an ingested red blood cell in the cytoplasm. The nuclear structure is still fairly typical although the karyosome is eccentrically located. Ingestion of red blood cells byE.

histolytica occurs in patients with invasive disease and is

considered to be a characteristic that distinguishes this organism from the other species ofEntamoeba. In addition to red cells, bacteria and cell debris can be found in the phagosomes of this parasite. Macrophages that have ingested cellular material can sometimes be confused with E. histolytica.

Amoebiasis Intestine
E. coli \u2013 Iodine Stained
E.coli trophozoite with nucleus
in
which
the
karyosome
appears large and irregular

E. coli trophozoite with nuclei in which the karyosome is eccentric,

cytoplasm
is
vacuolated
Entamoeba histolytica cyst
Entamoeba harmanii

Typical E. harmanii trophozoites are small, and characteristically take a light stain. The nucleus is small, has a discrete central karyosome, and fine peripheral chromatin. The cytoplasm, which usually is finely granular, never contains red blood cells.

Endolimax nana cyst
ENTAMOEBA HISTOLYTICA
ENTAMOEBA COLI
ENDOLIMAX NANA
ENTAMOEBA HARMANII

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