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Table Of Contents

PREFACE—COURSE DESCRIPTION
CHAPTER 1--MICROSCOPY
LIGHT MICROSCOPY
ELECTRON MICROSCOPY
MICROSCOPY APPENDIX. ALDEHYDE FIXATIVES
CHAPTER 2--SPECTROPHOTOMETRY
SPECTROPHOTOMETRY THEORY
INSTRUMENTATION
APPLICATIONS OF SPECTROPHOTOMETRY
FACTORS AFFECTING ABSORPTION
VARIATIONS IN SPECTROPHOTOMETRY
APPENDIX I. PHOTOMULTIPLIER TUBE
APPENDIX II. CALCULATION OF ENZYME ACTIVITY
CHAPTER 3--FLUORESCENCE
ENZYME ASSAYS AND FLUOROMETERS
FLUORESCENCE MICROSCOPY
FLOW CYTOMETRY
CHAPTER 4--RADIOCHEMISTRY
ATOMIC STRUCTURE AND RADIOACTIVE DECAY
PROPERTIES OF RADIOISOTOPES
MEASUREMENT OF ß-RADIATION
GAMMA-RAY DETECTION
SAFETY
APPENDIX 1. INTERNAL STANDARDS
APPENDIX 2. CHANNELS RATIO
APPENDIX 3. MULTIPLE ISOTOPES
CHAPTER 5--pH AND BUFFERS
DEFINITIONS
HENDERSON-HASSELBACH
COMPLICATIONS
BUFFERING CAPACITY
BUFFER SELECTION
BUFFER APPENDIX
CHAPTER 6--CENTRIFUGATION
SEDIMENTATION THEORY
PREPARATIVE CENTRIFUGATION
CENTRIFUGATION APPENDIX 1. RCF CALCULATION
CHAPTER 7--INTRODUCTION TO PROTEINS
PROTEIN STRUCTURE
PROTEIN STABILITY
PROTEIN ASSAYS
APPENDIX. AMINO ACIDS: PROPERTIES AND STRUCTURE
CHAPTER 8--DIFFERENTIAL PRECIPITATION OF PROTEINS
PROCEDURE FOR (NH4)2SO4 PRECIPITATION
OTHER PROTEIN PRECIPITATION METHODS
APPENDIX. AMMONIUM SULFATE NOMOGRAM
CHAPTER 9--CHROMATOGRAPHY
BASIC PRINCIPALS
EQUIPMENT
ADSORPTION CHROMATOGRAPHY
ION EXCHANGE CHROMATOGRAPHY
HYDROPHOBIC CHROMATOGRAPHY
GEL FILTRATION CHROMATOGRAPHY
AFFINITY CHROMATOGRAPHY
DETERGENTS
DETERGENT REMOVAL
APPENDIX 1. CELL DISRUPTION TECHNIQUES
CHAPTER 11--ELECTROPHORESIS
GEL ELECTROPHORESIS
SDS-PAGE
ISOELECTRIC FOCUSING
TWO-DIMENSIONAL GEL ELECTROPHORESIS
PROTEIN DETECTION FOLLOWING ELECTROPHORESIS
PREPARATIVE ELECTROPHORESIS
ELECTROPHORESIS APPENDIX 1. SIZE CALCULATION
ELECTROPHORESIS APPENDIX 2. GEL STAINING
IMMUNOGENICITY
IMMUNOGEN PREPARATION
IMMUNIZATION
COLLECTING AND PROCESSING BLOOD
ANTIBODY PURIFICATION
CHAPTER 14--MONOCLONAL ANTIBODIES
FUSION
SELECTION
SCREENING
CLONING
PRODUCTION
APPENDIX. B-CELL DIFFERENTIATION
CHAPTER 15--IMMUNOASSAYS
IMMUNOPRECIPITATION
IMMUNOBLOTTING
IMMUNOFLUORESCENCE
IMMUNOGOLD ELECTRON MICROSCOPY
ELISA (AND RIA)
DIRECT VS. INDIRECT
Direct vs. Indirect
OTHER METHODS
CHAPTER 16--NUCLEIC ACID STRUCTURE AND ISOLATION
ISOLATION OF NUCLEIC ACIDS
ISOLATION OF RNA
DENSITY GRADIENT CENTRIFUGATION
ANALYSIS AND QUANTIFICATION
RESTRICTION ENDONUCLEASES
CHAPTER 18--ELECTROPHORESIS OF NUCLEIC ACIDS
PULSE FIELD GEL ELECTROPHORESIS
CHAPTER 19--HYBRIDIZATION AND BLOTTING TECHNIQUES
GENERAL PROCEDURES
FACTORS AFFECTING HYBRIDIZATION
PREPARATION OF LABELED DNA PROBES
RFLP AND RESTRICTION MAPPING
IN SITU HYBRIDIZATION
DNA MICROARRAYS
CHAPTER 20--POLYMERASE CHAIN REACTION
PCR MECHANISM
PRACTICAL ASPECTS
RNA-PCR
QUANTITATIVE PCR
LIMITATIONS
PRECAUTIONS
Precautions
CHAPTER 21--RECOMBINANT DNA
PREPARATION OF FOREIGN DNA
PLASMID VECTORS
BACTERIOPHAGE λ
FILAMENTOUS BACTERIOPHAGE
COSMIDS AND YACS
QUALITY CONTROL
CHAPTER 22--EXPRESSION OF RECOMBINANT PROTEINS
EXPRESSION IN E. COLI
PREPARATION OF EXPRESSION VECTORS
SCREENING λ LIBRARIES WITH ANTIBODIES
EXPRESSION IN EUKARYOTES
APPENDIX 1. CODES AND CODONS
APPENDIX 2. FUSION PROTEINS
APPENDIX 3. GENERATING FRAMESHIFTS
CHAPTER 23--DNA SEQUENCING
DIDEOXY CHAIN TERMINATION
EXTENDED SEQUENCING STRATEGIES
Extended Sequencing Strategies
MAXIM AND GILBERT
CHAPTER 24--SEQUENCE ANALYSIS AND BIOINFORMATICS
SEQUENCE ALIGNMENT
SEARCHING DATABASES
PROTEOMICS
APPENDIX 1. WEBSITES
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Biotech Notes

Biotech Notes

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Published by babananak

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Published by: babananak on Oct 10, 2011
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