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Guide to SuccessfulOperation of Your LC System
34 Maple StreetMilford, MA 01757WAT022378TP, Revision 0
 
NOTICE 
The information in this document is subject to change without notice and should not beconstrued as a commitment by Waters Corporation. Waters Corporation assumes noresponsibility for any errors that may appear in this document. This manual is believed tobe complete and accurate at the time of publication. In no event shall Waters Corporationbe liable for incidental or consequential damages in connection with, or arising from, theuse of this manual. © 1991 WATERS CORPORATION. PRINTED IN THE UNITED STATES OF AMERICA.ALL RIGHTS RESERVED. THIS BOOK OR PARTS THEREOF MAY NOT BEREPRODUCED IN ANY FORM WITHOUT THE WRITTEN PERMISSION OF THEPUBLISHER.Waters is a registered trademark of Waters Corporation.Milli-Q is a registered trademark of Millipore CorporationAll other trademarks are the sole property of their respective owners.
 
Table of Contents3 
How to Use This Guide ......................................................................9Chapter 1Introduction to System Troubleshooting ...........................................131.1 Get the Facts..........................................................................141.2 Check Simple Things First.....................................................161.3 Compare System Performance to EstablishedBenchmarks..........................................................................171.4 Identify Possible Causes.......................................................191.5 Use a Systematic Troubleshooting Approach.......................211.6 Getting Help..........................................................................221.7 Troubleshooting References..................................................22Chapter 2Troubleshooting an LC System ........................................................232.1 Initial Survey of System Problems........................................252.2 Isolating System Problems...................................................292.2.1 System Pressure.......................................................292.2.2 Baseline Noise...........................................................392.2.3 Changes in Chromatographic Resolution orResults......................................................................67Chapter 3Troubleshooting System Components ...........................................1093.1 Pump Troubleshooting........................................................1103.1.1 Pump Troubleshooting Table....................................111
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