TITLE: EFFECT OF ENZYME CONCENTRATION ON THE RATE OF REACTION
To study and correlate what is the effect of enzyme on the rate of reaction. Thisexperiment used enzyme (smashed potato as catalyze) on rate of decomposition of Hydrogen Peroxide produce water and oxygen by measuring the initial rate of reaction.
To develop experimental and investigate skills.
What is the effect of enzyme concentration on the rate of reaction?
The higher the enzyme concentration used (smashed potato) the higher the rate of reactionuntil it reaches a point where the entire enzyme is saturated. Beyond this point, the rate of reaction remains the same, provided that the amount of substrate (Hydrogen Peroxide) used isconstant.
Enzyme concentration of catalase makes the reaction of decomposition of Hydrogen Peroxideslower.
Enzyme concentration ( no of spatula full of blended potato used )
Initial rate of reaction ( the gradient of the rate of reaction graph )
Volume of hydrogen peroxide, H
usedTemperature in which the experiment was conducted.
Beaker, graduated tube, bung stopper, conical flask, dropper, spatula, measuring cylinder,delivery tube, stopwatch.
Distilled water, buffer solution of pH 6.5, hydrogen peroxide, H
solution, blended potato,pair of gloves