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Effect of Enzyme Concentration of Rate of Reaction

Effect of Enzyme Concentration of Rate of Reaction

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Published by: Abdul Rahman Mohamed on Oct 11, 2011
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07/31/2013

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TITLE: EFFECT OF ENZYME CONCENTRATION ON THE RATE OF REACTION
OBJECTIVE
 
To study and correlate what is the effect of enzyme on the rate of reaction. Thisexperiment used enzyme (smashed potato as catalyze) on rate of decomposition of Hydrogen Peroxide produce water and oxygen by measuring the initial rate of reaction.
 
To develop experimental and investigate skills.
PROBLEM STATEMENT
What is the effect of enzyme concentration on the rate of reaction?
HYPOTHESIS
The higher the enzyme concentration used (smashed potato) the higher the rate of reactionuntil it reaches a point where the entire enzyme is saturated. Beyond this point, the rate of reaction remains the same, provided that the amount of substrate (Hydrogen Peroxide) used isconstant.
NULL HYPOTHESIS
Enzyme concentration of catalase makes the reaction of decomposition of Hydrogen Peroxideslower.
VARIABLESMANIPULATED :
Enzyme concentration ( no of spatula full of blended potato used )
RESPONDING :
Initial rate of reaction ( the gradient of the rate of reaction graph )
CONSTANT :
Volume of hydrogen peroxide, H
2
O
2
usedTemperature in which the experiment was conducted.
APPARATUS
Beaker, graduated tube, bung stopper, conical flask, dropper, spatula, measuring cylinder,delivery tube, stopwatch.
MATERIALS
Distilled water, buffer solution of pH 6.5, hydrogen peroxide, H
2
O
2
solution, blended potato,pair of gloves
 
 
Table 1.2: shows the results after 2.5cm
3
of hydrogen peroxide is placed into the conicalFlask containing 2 spatula of blended potato mixed with 5cm
3
buffer solutions.TIME,s Volume of oxygen gasproduced,cm
3
 0 010 1.820 3.830 6.040 8.050 9.560 11.870 13.680 15.690 18.6100 21.6110 23.6120 25.0130 26.6140 28.4150 28.6160 29.2170 29.6180 30.0190 30.6200 31.2210 31.4220 31.6230 32.0240 32.2250 32.8260 32.8270 32.8280 32.8290 32.8300 32.8
 
 - Graph 1.2 shows the volume of oxygen gas produced when 2.5cm
3
of hydrogen peroxide isplaced into the conical flask containing 2 spatula of blended potato mixed with 5cm3
 
buffersolutions.Initial rate of reaction = gradient of tangent = volume of oxygen gas collected ( cm
3
)Time (s)= 15.5 - 080 - 0= 0.19 cm
3
s
-1
 
 
05101520253035050100150200250300350
   V   O   L   U   M   E   O   F   O   X   Y   G   E   N   G   A   S   C   O   L   L   E   C   T   E   D ,   c   m
   3
 
TIMES,s
VOLUME OF OXYGEN GAS COLLECTED AGAINST TIME

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