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Peripheral Smear

Peripheral Smear

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Published by: api-3823785 on Oct 18, 2008
Copyright:Attribution Non-commercial

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03/18/2014

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Peripheral smear
Preparations
\u2022Specimen-direct capillary blood or
anticoagulated blood
\u2022Apparatus-clean glass slides,lancet,
spreader slide,microscope&microscope
oil
\u2022Stain-leishman stain,wright or gimsa
\u2022Diluent-distilled water or buffer solution
with Ph 6.8
Composition of leishman\u2019s stain

\u2022Polychromed methylene blue
\u2022Eosin azure leishman powder
\u2022Acetone free absolute alcohol
\u20221.5gms leishman powder in 1 liter

acetone free absolute alcohol

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