\ue000In vitro assays
\ue000Systems or disease models
\ue000Assays using biological tissues
\ue000Cell membranes,isolated whole cells,isolated tissues,isolated organs and whole animals are used.
\ue000Helps in identifying the Hits,Lead,and the Candidate drugs for further evaluation
\ue000Cell membrane fractions from organs or cultured cells---source of receptors
\ue000----for affinity and selectivity
\ue000E.g.Alpha adrenoceptors to evaluate binding of alpha agonists
\ue000Enzymes e.g.Tyrosine hydroxylase from sympathetic nerve endings---enzyme
\ue000Detect the cellular function-Affinity, selectivity and potency of the drug.
\ue000Effect on post-receptor mechanisms
\ue000Pharmacokinetics e.g.absorption across CaCo-2 cell lines
\ue000In-vitro metabolism Hep-G2 CELL lines
\ue000Hepatocytocytes and Kidney cell line-- toxicity
\ue000Drug- interaction studies
\ue000Effect on other organ systems
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