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P. 1
Lecture 1[1]

Lecture 1[1]

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Published by MuhammadFakhriAimi

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Published by: MuhammadFakhriAimi on Nov 10, 2011
Copyright:Attribution Non-commercial

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05/01/2012

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Learning Outcomes:
At the end of this lecture, students will be ableto:
define PCR
Describe components required for a PCRreaction and their function
Elaborate basics of PCR cycling
Tell how to check on PCR product
Explain factors affecting PCR reaction
Explain uses of PCR and their application in lifesciences
 
What is polymerase chain reaction
(PCR)?
It is a techniques used selectively to amplify aparticular segment of DNA
The segment may represent a small part of a largeand complex mixtures of DNAseg. Region A of the DNA molecule shown below
Acts like a molecular photocopier
 
The Invention of PCR
Invented by Kary Mullis in 1983
First published in 1985
Awarded Nobel Prize for Chemistry in 1993
Basic principle of replication a piece of DNAusing 2 primers was first described by GobindKhorana(1971) J. Mol. Biol. 56, 341-346
Progress was limited by primer synthesis andpolymerase purification issues
Mullis properly exploited amplification

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