This study investigated the effects of blue honeysuckle extract (BHE) on lipopolysaccharide-induced uveitis (EIU) in rats. Rats were injected with LPS to induce EIU and then treated with various doses of BHE. Treatment with BHE significantly reduced inflammatory cell infiltration in the aqueous humor, protein levels, and levels of nitric oxide, tumor necrosis factor-alpha, and prostaglandin E2. BHE also improved the histological status of ocular tissues and reduced the number of activated nuclear factor-kappa B cells. In addition, BHE treatment suppressed the production of inflammatory markers and expression of related enzymes in LPS-stimulated macrophage cells. The results suggest that
This study investigated the effects of blue honeysuckle extract (BHE) on lipopolysaccharide-induced uveitis (EIU) in rats. Rats were injected with LPS to induce EIU and then treated with various doses of BHE. Treatment with BHE significantly reduced inflammatory cell infiltration in the aqueous humor, protein levels, and levels of nitric oxide, tumor necrosis factor-alpha, and prostaglandin E2. BHE also improved the histological status of ocular tissues and reduced the number of activated nuclear factor-kappa B cells. In addition, BHE treatment suppressed the production of inflammatory markers and expression of related enzymes in LPS-stimulated macrophage cells. The results suggest that
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This study investigated the effects of blue honeysuckle extract (BHE) on lipopolysaccharide-induced uveitis (EIU) in rats. Rats were injected with LPS to induce EIU and then treated with various doses of BHE. Treatment with BHE significantly reduced inflammatory cell infiltration in the aqueous humor, protein levels, and levels of nitric oxide, tumor necrosis factor-alpha, and prostaglandin E2. BHE also improved the histological status of ocular tissues and reduced the number of activated nuclear factor-kappa B cells. In addition, BHE treatment suppressed the production of inflammatory markers and expression of related enzymes in LPS-stimulated macrophage cells. The results suggest that
Copyright:
Attribution Non-Commercial (BY-NC)
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Download as DOC, PDF, TXT or read online from Scribd
The aim of the present study was to investigate the
effects of blue honeysuckle extract (BHE), which contains high level of phenolic compounds, on endotoxin-induced uveitis (EIU). Male Lewis rats were randomly divided into 5 groups with 14 rats in each (eight rats for collection of aqueous humor, six rats for histologic examination). EIU was induced by a footpad injection of lipopolysaccharide (LPS). 1, 10, or 100 mg of BHE was injected intravenously immediately after LPS injection. The aqueous humor was collected at 24 h after LPS injection, the number of infiltrating cells, protein concentration, nitric oxide (NO), tumor necrosis factor (TNF)-a, and prostaglandin (PG)-E2 levels in the aqueous humor were determined. Some eyes were enucleated for histologic examination and immunohistochemical analysis. Immunohistochemical staining with a monoclonal antibody against activated nuclear factor (NF)-kB was performed to evaluate the effect of BHE on NF-kB activation. To further clarify the anti- inflammatory effect, RAW264.7 cells (a mouse macrophage cell line) were stimulated with LPS in the presence or absence of BHE and its major phenolics, cyanidin 3-glucoside (C3G), cyanidin 3-rutinoside (C3R), chlorogenic acid (CA). Expression of inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2) were analyzed by Western blot method. BHE treatment significantly reduced the inflammatory cell infiltration, the protein concentration, the levels of NO, TNF-a and PGE2 in the aqueous humor and improved histologic status of the ocular tissue. The number of activated NF-kB-positive cells was lower in the iris-ciliary body treated with BHE at 3 h after LPS injection. BHE significantly suppressed the production of NO, PGE2 and TNF-a in the culture medium as well as the expression of iNOS and COX-2 by LPS-stimulated RAW264.7 cells in a dose-dependent fashion. C3G, C3R and CA showed no or weak inhibitory effects on the level of inflammatory mediators and the expression of iNOS and COX-2. These results suggest that BHE attenuates the degree of inflammation in eyes with EIU by inhibiting the NF-kB dependent signaling pathway and the subsequent production of proinflammatory mediators.
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