Welcome to Scribd, the world's digital library. Read, publish, and share books and documents. See more
Download
Standard view
Full view
of .
Look up keyword
Like this
1Activity
0 of .
Results for:
No results containing your search query
P. 1
Husmhema Upt Stm Bm3

Husmhema Upt Stm Bm3

Ratings: (0)|Views: 73|Likes:
Published by zakwanmustafa

More info:

Published by: zakwanmustafa on Nov 20, 2011
Copyright:Attribution Non-commercial

Availability:

Read on Scribd mobile: iPhone, iPad and Android.
download as PDF, TXT or read online from Scribd
See more
See less

11/20/2011

pdf

text

original

 
HAEMATOLOGY DEPARTMENT, HOSPITAL UNIVERSITI SAINS MALAYSIATITLE :BONE MARROW: PRUSSIAN BLUEREACTION STAIN (PERLS’ STAIN)VERSION NO. 1PROCEDURE NO. HUSM/HEMA-UPT/STM-BM3 VERSION DATE. 24.03.2011
Page 1 of 3 STANDARD TECHNICAL MANUAL
APPROVED BY:
………
.
…………………………………
..ASSOC PROF DR ROSLINE HASSANHEAD OF HAEMATOLOGY DEPARTMENTCONTROLLED COPY NO : 3 REGISTERED HOLDERHAEMATOLOGY LABORATORYRECORD OF REVIEW/AMMENDMENTDATEVERSIONNO.DETAIL OF AMMENDMENT BY
 
HAEMATOLOGY DEPARTMENT, HOSPITAL UNIVERSITI SAINS MALAYSIATITLE :BONE MARROW: PRUSSIAN BLUEREACTION STAIN (PERLS’ STAIN)VERSION NO. 1PROCEDURE NO. HUSM/HEMA-UPT/STM-BM3 VERSION DATE. 24.03.2011
Page 2 of 3 STANDARD TECHNICAL MANUAL
PREPARED BY : ANG CHENG YONGDESIGNATION : TRAINING OFFICER / SCIENTIFIC OFFICERCHECKED BY : DR SHAFINI MOHAMED YUSOFFDESIGNATION : HAEMATOLOGISTAUTHORISED BY : ASSOC PROF DR ROSLINE HASSANDESIGNATION : HAEMATOLOGIST/LAB DIRECTOR
1. OBJECTIVE
To demonstrate the presence of iron in the bone marrow.
2. METHOD
Manually
3. PRINCIPLE
The reaction occurs with the treatment of bone marrow smear in acid solution of ferrocyanides. Any ferric ion (+3) in the smear with the ferrocyanide and results in theformation of a bright blue pigment called “Prussian blue” or ferric ferrocyanide.
4. REQUIREMENTS
4.1
EQUIPMENT
4.1.1
Staining Container 
4.1.2
Microscope slides and cover slips
4.2
REAGENT
4.2.1 2% Potassium Ferrocyanide (K
4
Fe(CN)
6
.3H
2
O)Pottasium Ferrocyanide 2 gDistilled Water 100 ml4.2.2 0.2N HCl (0.2 mol/L HCl)Distilled Water - 500 mlAcid Hydrochloride - 2.74 ml4.2.3 0.1% SafraninSafranin - 1gDistilled Water - 100 ml4.2.4 Absolute Methanol (Fixative)4.2.5 DPX (mountant)

You're Reading a Free Preview

Download
scribd
/*********** DO NOT ALTER ANYTHING BELOW THIS LINE ! ************/ var s_code=s.t();if(s_code)document.write(s_code)//-->