Description

The objective of this study was to develop and optimise a broad-range PCR protocol to detect and allow Gram-typing on bacterial 16S rDNA, and to provide bacterial identification by sequence analyses without the need for culture in a routine clinical diagnostic laboratory setting. The optimised PCR assays were tested on 233 clinical samples and the molecular results compared with culture. In conclusion, the broad-range PCR protocols developed can be applied for use in a routine clinical diagnostic laboratory, and together with sequencing analysis can enable bacterial identification.

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