ClLraLe

lsoclLraLe

keLogluLaraLe
Succlnyl CoA
SucclnaLe
lumaraLe
MalaLe
CxaloaceLaLe
7/. <-.s: _,-:.
~---+ x...-
Jere does lL occur?
W MaLrlx of mlLocondrla
Pow many pyruvaLes go Lroug lL per glucose?
W 1wo
JaL ls produced?
W nAuP lAuP
2
A1 and CC
2
efore enLerlng Le krebs Cycle pyruvaLe musL go
Lroug an lnlLlaLlon reacLlon Lo pass lnLo Le
mlLocondrla
CxaloaclLaLe + AceLyl CoA ClLraLe + CoA SP
1 CoA SP leaves mlLocondrla Lo blnd wlL
anoLer pyruvaLe
2 CxaloaclLaLe blnds wlL aceLyl group of aceLyl
CoA
3 ClLraLe ls produced
Lnzyme of reacLlon clLraLe synLase
ClLraLe lsoclLraLe + P
2
C
1 JaLer molecule ls removed from clLraLe
2 A second waLer molecule ls added Lo clLraLe
3 ClLraLes lsomer lsoclLraLe ls produced
Lnzyme of reacLlon aconlLase
lsoclLraLe + nAu
+
keLogluLaraLe + CC2 +
(nAuP + P
+
)
1 CC
2
molecule ls losL
2 8emalnlng compound ls oxldlzed wlL nAu
+
3 nAuP + P
+
ls reduced
4 keLogluLaraLe ls produced
Lnzyme of reacLlon lsoclLraLe deydrogenase
keLogluLaraLe + nAu
+
+ CoA SP Succlnyl CoA +
CC2 + (nAuP + P
+
)
1 CC
2
molecule ls losL
2 8emalnlng compound ls oxldlzed wlL nAu
+
3 nAuP + P
+
ls reduced
4 8emalnlng molecule blnds Lo CoA SP
3 Succlnyl CoA ls produced
Lnzyme of reacLlon keLogluLaraLe deydrogenase
SucclnylCoA + Cu +
l
SucclnaLe + CoASP +
C1
1 CoASP ls dlsplaced by a pospaLe group
2 ospaLe group ls Lransferred Lo Cu
3 Cu forms C1
4 SucclnaLe ls produced
Lnzyme of reacLlon succlnylCoA synLeLase
lormaLlon of A1 by SubsLraLe Level
osporllaLlon
SucclnaLe + lAu lumaraLe + lAuP
2
1 1wo ydrogen molecules are Lransferred Lo
lAu
2 lAuP
2
ls reduced
3 SucclnaLe ls oxldlzed
4 lumaraLe ls produced
Lnzyme of reacLlon succlnaLe deydrogenase
lumaraLe + P
2
C MalaLe
1 JaLer molecule ls added
2 onds of subsLraLe are rearranged
3 MalaLe ls produced
Lnzyme of reacLlon fumarase
MalaLe + nAu
+
CxaloaceLaLe +
(nAuP + P
+
)
1 SubsLraLe ls oxldlzed wlL nAu
+
2 nAuP + P
+
ls reduced
3 CxaloaceLaLe ls produced
Lnzyme of reacLlon malaLe deydrogenase
lor Lwo pyruvaLe (Lwo Lurns)
W 2 A1
W 6 nAuP + P
+
W 2 lAuP
2
+ P
+
W 6 CC
2
ender Pal ClLrlc Acld Cycle clockomos
commoolty colleqe ulstooce leotoloq
Clackamas CommunlLy College 2003
Jeb 29 nov 2011
LLp//dlclackamasedu/c106
06/clLrlcLm
CpardL Carles L ClLrlc Acld Cycle Summary
lmbotst colleqe lmbotst llllools LlmursL
College 2003 Jeb 28 nov 2011
LLp//wwwelmursLedu/cm/vcemboo
k/612clLrlcsumLml