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RADIAL IMMUNODIFFUSION
Objectives
Principle
Kit description
Standard antigen at different concentration (0.25 mg/ml, 0.5 mg/ml,
1.0mg/ml, 2.0mg/ml)
Materials
As listed in practical manual
Procedure
1. Agarose gel with antiserum is already prepared.
2. Punch the agarose gel into 6 well.
3. Each well added with standard antigen and
test antigen.
Wells:
I. Standard antigen A (0.25mg/ml)
II. Standard antigen B (0.5mg/ml)
III. Standard antigen C (1 .Omg/ml)
IV. Standard antigen D (2.Omg/ml)
V. Test antigen-1
VI. Test antigen-2
4.Keep in moist chamber. Incubate at RT overnight.
5.Diameter measured and graph plotted.
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[PRACTICAL 2] MTEB 2404
RESULTS
Table below showed the result from the Radial Immunodiffusion experiment:
STD Ag CONE (IN RING DIAMETER (IN
SAMPLE NUMBER
mg/ml) mm)
1 0.25 6
2 0.50 8
3 1.00 10
4 2.00 12
5 TEST SAMPLE 1 11.5
6 TEST SAMPLE 2 7
2
1
3 4
5
6
DISCUSSION
However, as it diffuses further and further from the well, its concentration
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[PRACTICAL 2] MTEB 2404
CONCLUSION
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